Closed system isolation and scalable expansion of human placental mesenchymal stem cells
| Autor: | M. Gunther, Michael R. Doran, Kerry Atkinson, Gary Brooke, Nicholas E. Timmins, Celena Heazlewood, Markus Kiel |
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| Rok vydání: | 2012 |
| Předmět: |
090302 Biomechanical Engineering
Computer science Placenta Cellular differentiation Bioreactor Cell Culture Techniques Bioengineering Cell Separation Regenerative Medicine Applied Microbiology and Biotechnology Regenerative medicine Cell therapy 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Pregnancy Humans Isolation (database systems) Cells Cultured Cell Proliferation Mesenchymal Stem/Stromal Cells business.industry Mesenchymal stem cell Cell Expansion Microcarrier Cell Differentiation Mesenchymal Stem Cells Biotechnology Cell biology Scalability 090301 Biomaterials Female business |
| Zdroj: | Biotechnology and Bioengineering |
| ISSN: | 0006-3592 |
| Popis: | Mesenchymal stem cells (MSC) are emerging as a leading cellular therapy for a number of diseases. However, for such treatments to become available as a routine therapeutic option, efficient and cost-effective means for industrial manufacture of MSC are required. At present, clinical grade MSC are manufactured through a process of manual cell culture in specialized cGMP facilities. This process is open, extremely labor intensive, costly, and impractical for anything more than a small number of patients. While it has been shown that MSC can be cultivated in stirred bioreactor systems using microcarriers, providing a route to process scale-up, the degree of numerical expansion achieved has generally been limited. Furthermore, little attention has been given to the issue of primary cell isolation from complex tissues such as placenta. In this article we describe the initial development of a closed process for bulk isolation of MSC from human placenta, and subsequent cultivation on microcarriers in scalable single-use bioreactor systems. Based on our initial data, we estimate that a single placenta may be sufficient to produce over 7,000 doses of therapeutic MSC using a large-scale process. |
| Databáze: | OpenAIRE |
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