Alterations in Synaptic Plasticity and Oxidative Stress Following Long-Term Paracetamol Treatment in Rat Brain
| Autor: | Tipthanan Chotipinit, Wilawan Ji-Au, Anan Srikiatkhachorn, Laddawan Lalert, Sirinapa Srikam, Supang Maneesri le Grand, Sompol Sanguanrungsirikul |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Time Factors Synaptic cleft Hippocampus Toxicology medicine.disease_cause Protein Carbonylation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Postsynaptic potential Internal medicine medicine Animals Neurochemistry Rats Wistar Acetaminophen Neuronal Plasticity biology General Neuroscience digestive oral and skin physiology Brain Glutathione Analgesics Non-Narcotic Rats Oxidative Stress 030104 developmental biology Endocrinology chemistry Synaptic plasticity Synapses Synaptophysin biology.protein 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Neurotoxicity research. 37(2) |
| ISSN: | 1476-3524 |
| Popis: | Several studies have recently revealed that cognitive function can be affected by paracetamol (APAP) treatment. However, the exact impact of this drug treatment on learning and memory has not been clarified. This study aimed to investigate the effect of APAP treatment on the alteration of synapses and oxidative stress in the rat frontal cortex and hippocampus. APAP at a dose of 200 mg/kg bw was fed to adult male Wistar rats through either acute (n = 10), 15-day (n = 10), or 30-day (n = 10) treatment regimens. The synaptic ultrastructure and proteins, synaptophysin (SYP) and postsynaptic density-95 (PSD-95), were monitored. The amount of protein carbonyl oxidation (PCO) and glutathione (GSH) levels were examined. Our results demonstrated that acute treatment with APAP had no effect on synapses and oxidative stress. However, the synapses obtained from rats with 15-day APAP treatment showed a marked shortening of active zones and widening of the synaptic cleft. Decrement of SYP and PSD-95 proteins were demonstrated in these rats as well. With 30-day APAP treatment, the alteration of the synaptic ultrastructure and proteins was more evident. Moreover, the depletion of GSH and the elevation of PCO levels were demonstrated in the rats treated with APAP for 30 days. These results suggest that long-term APAP treatment can induce synaptic degeneration in the hippocampus and frontal cortex. The increase in oxidative stress in these brain areas may be due to the deleterious effect of this drug. |
| Databáze: | OpenAIRE |
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