Protective Effect of Antioxidants in Nitric Oxide/COX-2 Interaction during Inflammatory Pain: The Role of Nitration
| Autor: | D. Ventrice, Valentina Malafoglia, Sara Ilari, Stefania Proietti, Fiorella Guadagni, Daniela Salvemini, Luigino Antonio Giancotti, Milena Rizzo, Ernesto Palma, Carolina Muscoli, Filomena Lauro, Micaela Gliozzi, Vincenzo Mollace, Antonella Spila, Concetta Dagostino |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry Prostaglandin lactate dehydrogenase (LDH) Inflammation Pharmacology reactive oxygen species (ROS) medicine.disease_cause Biochemistry Article Nitric oxide Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Molecular Biology Reactive nitrogen species prostaglandin E2 (PGE2) chemistry.chemical_classification Reactive oxygen species lcsh:RM1-950 Cell Biology nitration antioxidants 030104 developmental biology lcsh:Therapeutics. Pharmacology chemistry inflammation malondialdehyde (MDA) Hyperalgesia reactive nitrogen species (RNS) medicine.symptom cyclooxygenase 2 (COX-2) 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Antioxidants, Vol 9, Iss 1284, p 1284 (2020) Antioxidants Volume 9 Issue 12 |
| ISSN: | 2076-3921 |
| Popis: | In clinical practice, inflammatory pain is an important, unresolved health problem, despite the utilization of non-steroidal anti-inflammatory drugs (NSAIDs). In the last decade, different studies have proven that reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved in the development and maintenance of inflammatory pain and hyperalgesia via the post-translation modification of key proteins, such as manganese superoxide dismutase (MnSOD). It is well-known that inducible cyclooxygenase 2 (COX-2) plays a crucial role at the beginning of the inflammatory response by converting arachidonic acid into proinflammatory prostaglandin PGE2 and then producing other proinflammatory chemokines and cytokines. Here, we investigated the impact of oxidative stress on COX-2 and prostaglandin (PG) pathways in paw exudates, and we studied how this mechanism can be reversed by using antioxidants during hyperalgesia in a well-characterized model of inflammatory pain in rats. Our results reveal that during the inflammatory state, induced by intraplantar administration of carrageenan, the increase of PGE2 levels released in the paw exudates were associated with COX-2 nitration. Moreover, we showed that the inhibition of ROS with Mn (III) tetrakis (4-benzoic acid) porphyrin(MnTBAP) antioxidant prevented COX-2 nitration, restored the PGE2 levels, and blocked the development of thermal hyperalgesia. |
| Databáze: | OpenAIRE |
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