Continuous exposure of HeLa cells to caffeine
| Autor: | P. Himmelfarb, B.L. Carlson, P.S. Thayer, R.H. Liss |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Health
Toxicology and Mutagenesis Cell Cell Count Chemostat Chromatids Coffee HeLa Andrology chemistry.chemical_compound Caffeine Genetics medicine Humans Ingestion Continuous exposure Molecular Biology Chromosome Aberrations biology biology.organism_classification In vitro medicine.anatomical_structure Biochemistry chemistry Chromatid Cell Division HeLa Cells |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 12:197-203 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(71)90142-4 |
| Popis: | 2 techniques have been used with HeLa cells in vitro to determine whether there are cumulative effects of continuous exposure to caffeine at levels ranging from the maximum found in human blood after ingestion of 8 cups of coffee to several times higher. 3 experiments with cells growing in suspension in continuous (chemostat) culture for 7–11 days have shown no increase in incidence of chromatid breaks in the presence of caffeine at 5 and 20 μg/ml. In a second series of experiments, adherent cultures were continuously exposed to caffeine at 3 levels (5, 10 and 20 μg/ml) for 6 and 9 weeks (29 and 48 cell divisions), with subdivision of the cultures and examination of cells for chromatid breaks twice weekly. There were no significant effects on growth, as measured by overall increases in cell numbers, nor in chromatid breaks. Thus, long-term continuous exposure to caffeine at levels several times higher than the transitory peak levels which are normally attainable in man after ingestion of coffee, did not produce any evidence of increased chromatid breaks in 29–48 cell divisions. |
| Databáze: | OpenAIRE |
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