Preparation, characterization, and immunogenicity of conjugates composed of the O-specific polysaccharide of Shigella dysenteriae type 1 (Shiga's bacillus) bound to tetanus toxoid
| Autor: | Joseph Shiloach, Chiayung Chu, Rachel Schneerson, S S Szu, JohnB Robbins, Dolores A. Bryla, B K Liu, Douglas Watson |
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| Rok vydání: | 1991 |
| Předmět: |
Lipopolysaccharides
Shigellosis Magnetic Resonance Spectroscopy Shigella dysenteriae Immunology Monophosphoryl Lipid A Cross Reactions Microbiology Immunoglobulin G Shigella flexneri Mice Tetanus Toxoid medicine Animals Vaccines Synthetic biology Immune Sera Immunogenicity Polysaccharides Bacterial Toxoid medicine.disease biology.organism_classification Antibodies Bacterial Virology Bacterial vaccine Infectious Diseases Bacterial Vaccines Humoral immunity biology.protein Parasitology Research Article |
| Zdroj: | Infection and Immunity. 59:4450-4458 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | The background for developing conjugate vaccines for shigellosis composed of the O-specific polysaccharide (O-SP) bound to a protein is described elsewhere (C. Y. Chu, R. Schneerson, and J. B. Robbins, submitted for publication). Briefly, there is direct evidence for type (lipopolysaccharide [LPS])-specific protection after infection with the wild type or with attenuated strains of shigellae. Prospective studies of Israeli armed forces recruits show a correlation between preexisting serum immunoglobulin G (IgG) LPS antibodies and resistance to shigellosis (D. Cohen, M. S. Green, C. Block, R. Slephon, and I. Ofek, J. Clin. Microbiol. 29:386-389, 1991). In order to elicit IgG LPS-specific antibodies to Shigella dysenteriae type 1, the O-SP of this pathogen was purified and bound to tetanus toxoid (TT) by three schemes. The most immunogenic used a modification of a published method (C. Y. Chu, R. Schneerson, J. B. Robbins, and S. C. Rastogi, Infect. Immun. 40:245-256, 1983). The resultant O-SP-TT conjugates were stable and elicited high levels of IgG O-SP antibodies and booster responses in young mice when injected subcutaneously in saline at 1/10 the proposed human dose. Adsorption onto alum or concurrent administration with monophosphoryl lipid A enhanced both the IgG and IgM antibody responses to the O-SP of the conjugate; both the nonadsorbed and adsorbed conjugates elicited higher rises of IgG than of IgM antibodies. Clinical evaluations of S. dysenteriae type 1 O-SP-TT conjugates are planned. |
| Databáze: | OpenAIRE |
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