Molecular analysis of the TK locus in L5178Y large and small colony mouse lymphoma cell mutants induced by hycanthone methanesulfonate
| Autor: | Christine R. Hoffman, Jeffrey S. Dubins, Adel El-Tarras, Ronald R. Cobb, Janet Warner |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
biology
Lymphoma Health Toxicology and Mutagenesis Mutant Hycanthone Molecular biology Thymidine Kinase Restriction fragment chemistry.chemical_compound Mice chemistry Thymidine kinase Cell culture Mutation Genetics biology.protein Tumor Cells Cultured Animals Molecular Biology Gene DNA Cell Division Southern blot Mutagens |
| Zdroj: | Mutation research. 332(1-2) |
| ISSN: | 0027-5107 |
| Popis: | Mouse lymphoma cells of the L5178Y TK +/−3.7.2C line were exposed to varying concentrations of the anti-schistosomal drug hycanthone methanesulfonate. The trifluorothymidine (TFT)-resistant cells fell into two classes based on colony size. Southern blot analyses were performed using Nco I-digested DNA from a number of large and small mutant colonies from each treatment group. Two different restriction fragment banding patterns were identified in these analyses, those colonies that contained the 6.4 kb Nco I restriction fragment and those that did not. A total of 471 mutant colonies were analyzed and 84.5% (398) of these colonies did not exhibit the 6.4 kb fragment. There did not appear to be a hycanthone methanesulfonate dose response effect in the number of colonies that did not contain the 6.4 kb fragment among the treated groups. In addition, 82% (154 out of 188) of spontaneous mutants did not contain the 6.4 kb fragment. The results imply that greater than 80% of all spontaneous mutations found in the mouse lymphoma assay regardless of colony size do not contain the 6.4 kb fragment and each may be considered to be a large scale mutation. In addition, greater than 80% of the hycanthone induced mutations in the mouse lymphoma assay do not contain the 6.4 kb fragment and thus may be considered to be a large scale mutation. |
| Databáze: | OpenAIRE |
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