Upregulation of long non-coding RNA MYU promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells
| Autor: | Shu Zhang, Shudong Gu, Yan Liu, Li Qian, Hong Shen, Guoxin Mao, Jiefei Miao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Small interfering RNA proliferation Cell Vimentin 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) long non-coding RNA MYU medicine Gene silencing Gene knockdown biology Oncogene General Medicine Articles Cell cycle invasion Long non-coding RNA digestive system diseases esophageal squamous cell carcinoma 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Cancer research prognosis |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | Esophageal squamous cell carcinoma (ESCC) is a common malignant tumour type of the digestive system. Long non-coding RNA (lncRNA) c-Myc upregulated (MYU), also known as VPS9 domain-containing 1 antisense 1, was recently discovered. However, the expression of lncRNA MYU in ESCC and its role in tumour progression have remained elusive. In the present study, the expression of lncRNA MYU, Ki-67 and the epithelial-mesenchymal transition-related proteins E-cadherin and Vimentin in ESCC tissues was detected by reverse transcription-quantitative PCR. The expression of Ki-67, E-cadherin and Vimentin in ESCC tissues was also detected by immunohistochemistry. A small interfering RNA plasmid was employed to establish a TE-2 cell line with knockdown on lncRNA MYU. The results indicated that the expression of lncRNA MYU was higher in ESCC tissues than in normal adjacent tissues and that upregulation of lncRNA MYU was a potential biomarker for poor prognosis. The results also suggested that the expression levels of lncRNA MYU were correlated with the histological grade, lymph node metastasis and TNM stage (P |
| Databáze: | OpenAIRE |
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