Gamma-Delta T Lymphocytes in the Diagnostic Approach of Coeliac Disease
| Autor: | Petula Nijeboer, Gerd Bouma, Birgit I. Lissenberg-Witte, Tom van Gils, Rogier E. Ooijevaar, Chris J. J. Mulder, Hetty J. Bontkes, Martine Reijm |
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| Přispěvatelé: | Gastroenterology and hepatology, VU University medical center, Epidemiology and Data Science, APH - Methodology, Laboratory Medicine, AGEM - Endocrinology, metabolism and nutrition, AGEM - Digestive immunity, AGEM - Re-generation and cancer of the digestive system, AII - Infectious diseases |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Adolescent Duodenum T-Lymphocytes Biopsy chemical and pharmacologic phenomena Disease Sensitivity and Specificity digestive system Coeliac disease Flow cytometry Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans Intestinal Mucosa Child Receptor Intraepithelial Lymphocytes Aged Aged 80 and over medicine.diagnostic_test business.industry Gamma/Delta T-Lymphocyte fungi Gastroenterology Area under the curve hemic and immune systems Middle Aged Flow Cytometry medicine.disease Celiac Disease 030220 oncology & carcinogenesis Immunology Intraepithelial lymphocyte Female 030211 gastroenterology & hepatology business tissues |
| Zdroj: | Nijeboer, P, van Gils, T, Reijm, M, Ooijevaar, R, Lissenberg-Witte, B I, Bontkes, H J, Mulder, C J J & Bouma, G 2019, ' Gamma-delta T lymphocytes in the diagnostic approach of coeliac disease ', Journal of Clinical Gastroenterology, vol. 53, no. 5, pp. E208-E213 . https://doi.org/10.1097/MCG.0000000000001060, https://doi.org/10.1097/MCG.0000000000001060 Journal of Clinical Gastroenterology, 53(5), E208-E213. Lippincott Williams and Wilkins |
| ISSN: | 0192-0790 |
| DOI: | 10.1097/mcg.0000000000001060 |
| Popis: | Goals: To validate cut-off values of CD 3+ T-cell receptor gammadelta chain (TCRγδ + ) intraepithelial lymphocyte (IEL) in the (differential) diagnosis of celiac disease (CD). Background: CD is characterized by an increase in gamma-delta IEL (CD 3+ TCRγδ + IEL). Study: Percentages were determined by flow cytometric analysis of IELs from small bowel biopsies in 213 CD and 13 potential CD (PCD) patients and in total 112 controls. A cut-off value for percentages of CD 3+ TCRγδ + IEL to differentiate active CD and controls was obtained from a receiver operating characteristic curve and implemented in controls and PCD patients. Results: Percentage of CD 3+ TCRγδ + IEL was significantly increased in the majority of CD patients, irrespective of the presence of villous atrophy. A cut-off value of 14% for CD 3+ TCRγδ + IEL resulted in 66.3% sensitivity and 96.6% specificity for CD diagnosis (area under the curve, 88.6%). Conclusions: A percentage of ≥14% CD 3+ TCRγδ + IEL has a high specificity for CD diagnosis and can be of diagnostic help in cases where diagnosis is not straightforward. |
| Databáze: | OpenAIRE |
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