Mitochondrial oxidative stress elicits chromosomal instability after exposure to isocyanates in human kidney epithelial cells
| Autor: | Hemant Pandey, Hariom Panwar, Kewal K. Maudar, Gorantla V. Raghuram, Pradyumna Kumar Mishra, Deepika Jain |
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| Rok vydání: | 2009 |
| Předmět: |
Genome instability
Senescence Glutathione reductase Centromere Biology Mitochondrion medicine.disease_cause Kidney Biochemistry Cell Line Superoxide dismutase Chromosome instability Chromosomal Instability medicine Chromosomes Human Humans Antigens Cell Shape Cellular Senescence Chromosome Aberrations Superoxide Dismutase Cell Cycle Deoxyguanosine Epithelial Cells General Medicine Molecular biology Mitochondria Oxidative Stress Glutathione Reductase Gene Expression Regulation 8-Hydroxy-2'-Deoxyguanosine biology.protein Reactive Oxygen Species Cell aging Oxidative stress Isocyanates |
| Zdroj: | Free radical research. 43(8) |
| ISSN: | 1029-2470 |
| Popis: | The role of oxidative stress is often attributed in environmental renal diseases. Isocyanates, a ubiquitous chemical group with diverse industrial applications, are known to undergo bio-transformation reactions upon accidental and occupational exposure. This study delineates the role of isocyanate-mediated mitochondrial oxidative stress in eliciting chromosomal instability in cultured human kidney epithelial cells. Cells treated with 0.005 microM concentration of methyl isocyanate displayed morphological transformation and stress-induced senescence. Along the time course, an increase in DCF fluorescence indicative of oxidative stress, depletion of superoxide dismutase (SOD) and glutathione reductase (GR) and consistent accumulation of 8-oxo-dG were noticed. Thus, endogenous oxidative stress resulted in aberrant expression of p53, p21, cyclin E and CDK2 proteins, suggestive of deregulated cell cycle, chromosomal aberrations, centromeric amplification, aneuploidy and genomic instability. |
| Databáze: | OpenAIRE |
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