DNA-assisted selective electrofusion (DASE) of Escherichia coli and giant lipid vesicles
| Autor: | Pietro Cicuta, Lorenzo Di Michele, Sho Takamori, Shoji Takeuchi |
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| Přispěvatelé: | Commission of the European Communities, The Royal Society, Takamori, Sho [0000-0002-8892-2899], Cicuta, Pietro [0000-0002-9193-8496], Takeuchi, Shoji [0000-0001-6946-0409], Di Michele, Lorenzo [0000-0002-1458-9747], Apollo - University of Cambridge Repository |
| Rok vydání: | 2022 |
| Předmět: |
Technology
MEDIATED FUSION Chemistry Multidisciplinary Materials Science LIPOSOMES EFFICIENT Materials Science Multidisciplinary DETERMINANTS MEMBRANE-FUSION Physics Applied DELIVERY 10 Technology Escherichia coli General Materials Science Nanoscience & Nanotechnology Membranes Science & Technology PROGRAMMABLE FUSION 02 Physical Sciences Physics DNA Lipids Nanostructures GENOME Chemistry Physical Sciences CELLS Science & Technology - Other Topics SNARE PROTEIN 03 Chemical Sciences |
| Popis: | Synthetic biology and cellular engineering require chemical and physical alterations, which are typically achieved by fusing target cells with each other or with payload-carrying vectors. On one hand, electrofusion can efficiently induce the merging of biological cells and/or synthetic analogues via the application of intense DC pulses, but it lacks selectivity and often leads to uncontrolled fusion. On the other hand, synthetic DNA-based constructs, inspired by natural fusogenic proteins, have been shown to induce a selective fusion of artificial membranes, albeit with low efficiency. Here we introduce DNA-assisted selective electrofusion (DASE) which relies on membrane-anchored DNA constructs to bring together the objects one seeks to merge, and applying an electric impulse to trigger their fusion. The DASE process combines the efficiency of standard electrofusion and the selectivity of fusogenic nanostructures, as we demonstrate by inducing and characterizing the fusion of spheroplasts derived from Escherichia coli bacteria with cargo-carrying synthetic lipid vesicles. |
| Databáze: | OpenAIRE |
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