Mouse strain determines the outcome of wound healing after myocardial infarction
| Autor: | Jack P.M. Cleutjens, Ben J. A. Janssen, H. T. M. Vervoort‐Peters, Jos F.M. Smits, W. Matthijs Blankesteijn, Mat J.A.P. Daemen, Agnieszka Strzelecka, Susanne W.M. van den Borne, Veerle A.M. van de Schans, Peter Lijnen |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 2009 |
| Předmět: |
Cardiac function curve
Male medicine.medical_specialty Pathology Time Factors Physiology Heart Rupture Myocardial Infarction Blood Pressure Polymerase Chain Reaction Gene Expression Regulation Enzymologic Ventricular Function Left Mice Species Specificity Physiology (medical) Internal medicine Natriuretic Peptide Brain medicine Ventricular Pressure Animals Zymography Myocardial infarction RNA Messenger Antihypertensive Agents Heart Rupture Post-Infarction Inflammation Mice Inbred BALB C Wound Healing Ejection fraction business.industry Myocardium Stroke Volume medicine.disease Matrix Metalloproteinases Mice Inbred C57BL Disease Models Animal Echocardiography Heart failure Circulatory system Cardiology Cardiology and Cardiovascular Medicine Wound healing business Atrial Natriuretic Factor |
| Zdroj: | Cardiovascular research, 84(2), 273-282. Oxford University Press |
| ISSN: | 1755-3245 0008-6363 |
| Popis: | Aims Our objective was to study the effect of the genetic background on the wound healing process after myocardial infarction (MI) in mice. Methods and results MI was induced in five different mouse strains (BalbC, C57Bl6, FVB, 129S6, and Swiss). At 3, 14, and 28 days after MI, cardiac dimensions were monitored by echocardiography and histology, whereas cardiac function was determined by direct intraventricular pressure measurements (d P /d t ). Furthermore, matrix metalloproteinases were measured by zymography, and mRNA expression by quantitative PCR. Infarct rupture, which typically occurred at 3–6 days post-MI, was most frequent in 129S6 mice (62%), followed by C57Bl6 (36%), FVB (29%), Swiss (23%), and BalbC (5%). The high incidence of infarct rupture in 129S6 mice was associated with high systolic blood pressure and increased influx of inflammatory cells. Cardiac dilatation was most marked in Swiss mice and least prominent in 129S6 mice. The degree of dilatation was associated with a reduced ejection fraction and decreased d P /d t values at 14 and 28 days post-MI. At day 14 and 28 post-MI, secondary thinning of the infarct area was marked in BalbC, FVB, and Swiss, but absent in C57Bl6 and 129S6 mice. In the latter two groups, this was paralleled by the highest number of myofibroblasts at day 14 post-MI. Conclusion The outcome of infarct healing in mice strongly depends on genetic background. On the basis of our results, we suggest that for studies on infarct rupture, the 129S6 mouse is the background of choice, whereas BalbC and Swiss mice are the preferred models to study infarct thinning post-MI. |
| Databáze: | OpenAIRE |
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