Performance evaluation study of ID RHD XT, a new genotyping assay for the detection of high-prevalence RhD negative and weak D types

Autor:	Ellen van der Schoot, Miguel Ángel Vesga, Barbera Veldhuisen, Maria Azkarate, Diego Tejedor, Araitz Molano, Mercedes Piedrabuena, Mónica López, Izaskun Apraiz, Fernando Puente, Patricia España, Montserrat Rubia
Rok vydání:	2018
Předmět:	High prevalence Rh-Hr Blood-Group System Genotyping Techniques business.industry Integrin beta3 Hematology General Medicine 030204 cardiovascular system & hematology Virology Serology 03 medical and health sciences Call rate 0302 clinical medicine System failure RhD negative Genotype Medicine Humans Antigens Human Platelet Typing business Genotyping Alleles 030215 immunology
Zdroj:	Vox sanguinis. 113(7)
ISSN:	1423-0410
Popis:	BACKGROUND AND OBJECTIVES Routine serologic D typing does not distinguish between weak D subtypes and partial D phenotypes. The goal of this study was to validate the performance of the ID RHD XT genotyping assay. MATERIAL AND METHODS Previously serotyped samples for D antigen (n = 1000; 16% weak D serotyped donors) were analysed. The reference methods used for comparison were licensed serology tests for D antigen phenotype, and bidirectional sequencing (BDS) for weak D type confirmation and HPA-1 phenotype prediction. Discrepancies were solved with BDS and BLOODchip® Reference. RESULTS There were no system failure, a 100% call rate and no inconclusive results. ID RHD XT correctly called all (88/88) weak D types 1, 2 and 3. Review of other 87 apparent discrepancies identified a small number of serology errors and showed that ID RHD XT correctly signalled the presence of other RHD variants which were further confirmed by BDS and BLOODchip® Reference. The predicted HPA-1 phenotype by ID RHD XT was 100% concordant with BDS. CONCLUSION ID RHD XT genotype predictions for high-prevalence RhD negative and weak D types 1, 2 and 3 as well as for HPA-1a/HPA-1b antigens were accurate, which is of clinical significance in guiding transfusion needs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ff219bc664d6c17ea5309ac024fc34b https://pubmed.ncbi.nlm.nih.gov/30125050 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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