Dendritic cells prevent rather than promote immunity conferred by a helicobacter vaccine using a mycobacterial adjuvant
| Autor: | Mathias Oertli, Burkhard Becher, Anne Müller, Else Marie Agger, Iris Hitzler |
|---|---|
| Přispěvatelé: | University of Zurich, Müller, Anne |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Time Factors
medicine.medical_treatment Administration Oral 10263 Institute of Experimental Immunology T-Lymphocytes Regulatory Mice 0302 clinical medicine Cell Wall B-Lymphocytes Immunity Cellular 0303 health sciences biology Chemotaxis Stomach 10061 Institute of Molecular Cancer Research Gastroenterology T-Lymphocytes Helper-Inducer Antibodies Bacterial 3. Good health Vaccination medicine.anatomical_structure Bacterial Vaccines Antibody Adjuvant Injections Intraperitoneal Cholera Toxin Regulatory T cell Injections Subcutaneous Mice Transgenic 610 Medicine & health Cancer Vaccines Helicobacter Infections Mycobacterium 03 medical and health sciences Adjuvants Immunologic Stomach Neoplasms Immunity Immune Tolerance medicine Animals 2715 Gastroenterology Administration Intranasal 030304 developmental biology Helicobacter pylori Hepatology Dendritic Cells Dendritic cell biology.organism_classification Immunity Humoral Mice Inbred C57BL Immunization Immunology biology.protein 570 Life sciences 2721 Hepatology 030215 immunology |
| Zdroj: | Gastroenterology |
| Popis: | Background & Aims Immunization against the gastric bacterium Helicobacter pylori could prevent many gastric cancers and other disorders. Most vaccination protocols used in preclinical models are not suitable for humans. New adjuvants and a better understanding of the correlates and requirements for vaccine-induced protection are needed to accelerate development of vaccines for H pylori . Methods Vaccine-induced protection against H pylori infection and its local and systemic immunological correlates were assessed in animal models, using cholera toxin or CAF01 as adjuvants. The contribution of B cells, T-helper (Th)–cell subsets, and dendritic cells to H pylori –specific protection were analyzed in mice. Results Parenteral administration of a whole-cell sonicate, combined with the mycobacterial cell-wall–derived adjuvant CAF01, protected against infection with H pylori and required cell-mediated, but not humoral, immunity. The vaccine-induced control of H pylori was accompanied by Th1 and Th17 responses in the gastric mucosa and in the gut-draining mesenteric lymph nodes; both Th subsets were required for protective immunity against H pylori . The numbers of memory CD4 + T cells and neutrophils in gastric tissue were identified as the best correlates of protection. Systemic depletion of dendritic cells or regulatory T cells during challenge infection significantly increased protection by overriding immunological tolerance mechanisms activated by live H pylori . Conclusions Parenteral immunization with a Helicobacter vaccine using a novel mycobacterial adjuvant induces protective immunity against H pylori that is mediated by Th1 and Th17 cells. Tolerance mechanisms mediated by dendritic cells and regulatory T cells impair H pylori clearance and must be overcome to improve immunity. |
| Databáze: | OpenAIRE |
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