First detection of Israeli acute paralysis virus (IAPV) in France, a dicistrovirus affecting honeybees (Apis mellifera)
| Autor: | Olivier Celle, Richard Thiéry, Magali Ribière, Nicolas Cougoule, Jean-Paul Faucon, Philippe Blanchard, Patrick Drajnudel, Frank M. Schurr |
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| Přispěvatelé: | Laboratoire d'études et de recherches sur les petits ruminants et les abeilles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) |
| Rok vydání: | 2008 |
| Předmět: |
Veterinary medicine
Apiary Molecular Sequence Data Pcr cloning Insect Viruses Picornaviridae Disease cluster Colony collapse disorder Animals Winter losses Israel Phylogeny Ecology Evolution Behavior and Systematics Picornaviridae Infections [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Base Sequence Apidae biology Reverse Transcriptase Polymerase Chain Reaction Ecology Bees biology.organism_classification Apoidea RT-PCR detection Host-Pathogen Interactions [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Israeli acute paralysis virus (IAPV) RNA Viral Israeli acute paralysis virus Causal link France Apis mellifera |
| Zdroj: | Journal of Invertebrate Pathology Journal of Invertebrate Pathology, Elsevier, 2008, 99 (3), pp.348-50. ⟨10.1016/j.jip.2008.07.006⟩ |
| ISSN: | 0022-2011 1096-0805 |
| DOI: | 10.1016/j.jip.2008.07.006 |
| Popis: | International audience; Bee samples were collected in French apiaries that displayed severe losses and mortality during the winter (from November 2007 to March 2008). They were screened for the presence of Israeli acute paralysis virus (IAPV) by using RT-PCR. Five out of 35 surveyed apiaries, located in two different geographical areas, were found positive. This represents the first reported detection of IAPV in France. The specificity of the PCR products was checked by sequencing. The phylogenetic analysis showed that French isolates of IAPV were closely related to a cluster including American and Australian isolates. Nevertheless, most of American isolates previously reported to be associated to Colony Collapse Disorder (CCD) and an Israeli isolate first isolated in 2004 from dead bees were included in another cluster. Since IAPV was detected in only 14% of the affected apiaries, it was not possible to establish a causal link between IAPV and the severe winter losses that occurred. |
| Databáze: | OpenAIRE |
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