Simultaneous Determination and Risk Assessment of Pyrrolizidine Alkaloids in Artemisia capillaris Thunb. by UPLC-MS/MS Together with Chemometrics

Autor:	Huijuan Wang, Junchi Wang, Jianyong Si, Yong-Hong Liao, Yue Qiao, Qi-Lei Guo, Di-An Sun, Li-Hua Chen
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	High selectivity Pharmaceutical Science Artemisia capillaris Biology 01 natural sciences Analytical Chemistry Chemometrics lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound pyrrolizidine alkaloids lcsh:Organic chemistry Artemisia capillaris Thunb Drug Discovery PCX solid-phase extraction Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences Traditional medicine 010401 analytical chemistry Organic Chemistry risk assessment Asteraceae biology.organism_classification chemometrics 0104 chemical sciences Risk evaluation chemistry Chemistry (miscellaneous) UPLC-MS/MS Pyrrolizidine Molecular Medicine Uplc ms ms Risk assessment
Zdroj:	Molecules, Vol 24, Iss 6, p 1077 (2019) Molecules Volume 24 Issue 6
ISSN:	1420-3049
Popis:	Pyrrolizidine alkaloids (PAs) are natural toxins found in some genera of the family Asteraceae. However, it has not been reported whether PAs are present in the widely used Asteraceae plant Artemisia capillaris Thunb. (A. capillaris). The purpose of this study was to establish a sensitive and rapid UPLC-MS/MS method together with chemometrics analysis for simultaneous determination and risk assessment of PAs in A. capillaris. The developed UPLC-MS/MS method was validated and was confirmed to display desirable high selectivity, precision and accuracy. Risk assessment was conducted according to the European Medicines Agency (EMA) guideline. Chemometrics analysis was performed with hierarchical clustering analysis and principal component analysis to characterize the differences between PAs of A. capillaris. Finally, PAs were found in 29 out of 30 samples and at least two were detected in each sample, besides, more than half of the samples exceeded the EMA baseline. Nevertheless, the chemometrics results suggested that the PAs contents of A. capillaris from different sources varied significantly. The method was successfully applied to the detection and risk evaluation of PAs-containing A. capillaris for the first time. This study should provide a meaningful reference for the rational and safe use of A. capillaris.
Databáze:	OpenAIRE
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