Multicenter comparison of levels of antibody to the Neisseria meningitidis group A capsular polysaccharide measured by using an enzyme-linked immunosorbent assay
| Autor: | C E Frasch, Linda L. Gheesling, W F Bibb, S H Turner, Wallis E. DeWitt, Leta O. Helsel, Brian D. Plikaytis, George M. Carlone, Sally A. Quataert, G R Siber |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Adult Enzyme-Linked Immunosorbent Assay Meningococcal vaccine Neisseria meningitidis medicine.disease_cause Binding Competitive Microbiology Microtiter plate Antigen medicine Humans Multicenter Studies as Topic Antigens Bacterial medicine.diagnostic_test biology Immunogenicity Polysaccharides Bacterial Bacterial polysaccharide Virology Antibodies Bacterial Immunoassay biology.protein Binding Sites Antibody Antibody Research Article |
| Popis: | There is no standard immunoassay for evaluating immune responses to meningococcal vaccines. We developed an enzyme-linked immunosorbent assay to measure total levels of antibody to Neisseria meningitidis group A capsular polysaccharide. Five laboratories measured the antibody levels in six paired pre- and postvaccination serum samples by using the enzyme-linked immunosorbent assay. Methylated human serum albumin was used to bind native group A polysaccharide to microtiter plate surfaces. The between-laboratory coefficients of variation for pre- and postvaccination sera had ranges of 31 to 91 and 17 to 31, respectively. The mean laboratory coefficients of variation for pre- and postvaccination sera, respectively, were 17 and 11 (Molecular Biology Laboratory, Centers for Disease Control), 12 and 15 (Immunodiagnostic Methods Laboratory, Centers for Disease Control), 22 and 19 (Dana-Farber Cancer Institute), 38 and 38 (Bacterial Polysaccharide Laboratory, U.S. Food and Drug Administration), and 11 and 10 (Praxis Biologics, Inc.). Standardization of this enzyme-linked immunosorbent assay should allow interlaboratory comparison of meningococcal vaccine immunogenicity, thus providing a laboratory-based assessment tool for evaluating meningococcal vaccines. |
| Databáze: | OpenAIRE |
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