Comparative Analysis of Long Noncoding RNA Expression in Human Hepatocyte Cell Lines and Liver
| Autor: | A V Metelin, Dmitry A. Skvortsov, Olga A. Dontsova, Olga Y. Burenina, Timofei S. Zatsepin, E F Kim, M. P. Rubtsova |
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| Rok vydání: | 2020 |
| Předmět: |
Carcinoma
Hepatocellular Biophysics Biology medicine.disease_cause Biochemistry 03 medical and health sciences Gene expression medicine Humans RNA Messenger Cells Cultured 030304 developmental biology 0303 health sciences 030302 biochemistry & molecular biology Liver Neoplasms General Chemistry General Medicine Hep G2 Cells medicine.disease Long non-coding RNA In vitro Human hepatocyte medicine.anatomical_structure Liver Cell culture Hepatocyte Cancer research Hepatocytes RNA Long Noncoding Carcinogenesis Liver cancer |
| Zdroj: | Doklady. Biochemistry and biophysics. 493(1) |
| ISSN: | 1608-3091 |
| Popis: | Long noncoding RNAs (lncRNAs) are promising biomarkers and potential targets for liver cancer therapy. Stable hepatocyte lines are used in vitro to investigate functions of lncRNAs which amount in cell fluctuates during carcinogenesis. For the first time we compared gene expression of known lncRNAs in human conditional normal liver cells HepaRG and cancer cell lines Huh7 and HepG2. We showed that relative amounts of these lncRNAs in HepaRG are close to analogous variables measured for liver samples from healthy donors. Obtained data demonstrate exclusive peculiarities of HepaRG and confirm its reasonable application as a model of normal human hepatocytes for studying functions of lncRNAs. |
| Databáze: | OpenAIRE |
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