NMR spectroscopic properties (1H at 500 MHz) of deuterated∗ ribonucleotide-dimers ApU∗, GpC∗, partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗, d(ApT∗, d(GpC∗ and their comparison with natural counterparts (1H-NMR window)
| Autor: | András Földesi, Jyoti Chattopadhyaya, C. Glemarec, F. P. R. Nilson, C. Gioeli |
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| Rok vydání: | 1993 |
| Předmět: |
Magnetic Resonance Spectroscopy
Ribonucleotide Molecular Structure Chemistry Stereochemistry Chemical shift Deoxyribonucleotides Biophysics Ribonucleotides Deuterium Biochemistry Deoxyribonucleotide chemistry.chemical_compound Crystallography Biopolymers Kinetic isotope effect Proton NMR Molecule Protons Spectroscopy |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 26:1-26 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/0165-022x(93)90018-j |
| Popis: | Pure 1'#,2',3',4'#,5',5''-2H6-ribonucleoside derivatives 10-14, 1'#,2',2'',3',4'#,5',5''-2H7-2'-deoxynucleoside blocks 15-18 and their natural-abundance counterparts were used to assemble partially deuterated ribonucleotide-dimers (* indicates deuteration at 1'#,2',3',4'#,5',5''(2H6)): ApU* 21, GpC* 22 and partially deuterated 2'-deoxyribonucleotide-dimers d(TpA*) 23, d(ApT*) 25, d(GpC*) 26 (* indicates deuteration at 1'#,2',2'',3',4'#,5',5''(2H7)) according to the procedure described by Földesi et al. (Tetrahedron, in press). These five partially deuterated oligonucleotides were subsequently compared with their corresponding natural-abundance counterparts by 500 MHz 1H-NMR spectroscopy to evaluate the actual NMR simplifications achieved in the non-deuterated part (1H-NMR window) as a result of specific deuterium incorporation. Detailed one-dimensional 1H-NMR (500 MHz), two-dimensional correlation spectra (DQF-COSY and TOCSY) and deuterium isotope effect on the chemical shifts of oligonucleotides have been presented. |
| Databáze: | OpenAIRE |
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