Brazil’s first free-mating laboratory colony of Nyssorhynchus darlingi

Autor: Marta Moreno, Maisa da Silva Araujo, Paula Frassinetti Medeiros de Paulo, Glaucilene da Silva Costa, Dhelio Batista Pereira, Jansen Fernandes Medeiros, Luiz Hidelbrando Pereira-da-Silva, Alice Oliveira Andrade, Najara Akira Costa dos Santos, Carlos Tong Rios
Jazyk: angličtina
Předmět:
0301 basic medicine
Oviposition
LIGHT STIMULATION
pupation
0302 clinical medicine
Nyssorhynchus
Nyssorhynchus darlingi
animal
Anopheles darlingi
Mating
Malaria vector
media_common
copulation
Larva
biology
Reproduction
adult
Plasmodium vivax malaria
Anopheles
Anopheles/growth & development/physiology
mating
Pupa
Infectious Diseases
female
organism colony
pupa
Colony establishment
egg laying
mosquito vector
purl.org/pe-repo/ocde/ford#3.03.06 [https]
Brazil
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
media_common.quotation_subject
030231 tropical medicine
030106 microbiology
species identification
malaria
Zoology
Mosquito Vectors
Article
Mosquito Vectors/growth & development/physiology
reproduction
03 medical and health sciences
larva
male
stomatognathic system
parasitic diseases
Animals
sporozoite
DNA barcoding
growth
development and aging

nonhuman
biology.organism_classification
Malaria
genetic variation
physiology
Parasitology
microflora
laboratory
Zdroj: Revista da Sociedade Brasileira de Medicina Tropical, Vol 52
Revista da Sociedade Brasileira de Medicina Tropical, Volume: 52, Article number: e20190159, Published: 18 JUL 2019
ISSN: 1678-9849
0037-8682
Popis: INTRODUCTION: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil. METHODS: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation. RESULTS: In 11 generations, the colony produced a high number of pupae after the F5 generation. CONCLUSIONS: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.
Databáze: OpenAIRE