New variant groups identified from HGV isolates
| Autor: | Kazuhiko Katayama, Shuetsu Fukushi, Hirofumi Okamura, Fuminori B. Hoshino, Chie Kurihara, Ishiyama N, A Oya |
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| Rok vydání: | 1997 |
| Předmět: |
Genetics
Base Sequence Genotype Molecular epidemiology biology viruses Flaviviridae Molecular Sequence Data Nucleic acid sequence Genetic Variation virus diseases Sequence alignment General Medicine biology.organism_classification medicine.disease Virology GB virus C Virus medicine Humans Viral hepatitis Genotyping |
| Zdroj: | Archives of Virology. 142:1021-1028 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | We have determined the primary sequence of the 5' noncoding region (5' NCR) and putative helicase regions (NS-3) of hepatitis G virus (HGV) and GB virus C (GBV-C) that were isolated in Japan from suspected cases of nonA-nonB and/or nonA-nonB-nonC viral hepatitis by using RT-PCR, and we compared the newly isolated sequences with three established isolates. The addition of a "G" residue was found at the 5' terminus of all 8 Japanese isolates. These isolates were more clearly distinguished from the prototype viruses by comparison with the 5' NCR sequence than by comparison with the NS-3 region. Our results suggested that at least three distinct genomic variants of HGV exist. Genotyping of HGV by using RT-PCR based on the sequence of the 5' NCR seems highly feasible. |
| Databáze: | OpenAIRE |
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