Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva
| Autor: | Yuri Chaban, Michael A. Staniewski, Steven M. Short, S.F. Mirza, Cindy M. Short, Andrew M. Long |
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| Rok vydání: | 2015 |
| Předmět: |
Canada
food.ingredient viruses Molecular Sequence Data Genome Viral Haptophyte Evolution Molecular Algal virus food Genome Size Virology Phycodnaviridae Mimiviridae Prymnesiovirus Genome size Phylogeny Genetics Mimivirus biology Haptophyta biology.organism_classification Lakes Capsid Lytic cycle NCLDV Capsid Proteins |
| Zdroj: | Virology. 486 |
| ISSN: | 1096-0341 |
| Popis: | Water samples from Lake Ontario, Canada were tested for lytic activity against the freshwater haptophyte algae Chrysochromulina parva. A filterable lytic agent was isolated and identified as a virus via transmission electron microscopy and molecular methods. The virus, CpV-BQ1, is icosahedral, ca. 145nm in diameter, assembled within the cytoplasm, and has a genome size of ca. 485kb. Sequences obtained through PCR-amplification of DNA polymerase (polB) genes clustered among sequences from the family Phycodnaviridae, whereas major capsid protein (MCP) sequences clustered among sequences from either the Phycodnaviridae or Mimiviridae. Based on quantitative molecular assays, C. parva׳s abundance in Lake Ontario was relatively stable, yet CpV-BQ1׳s abundance was variable suggesting complex virus-host dynamics. This study demonstrates that CpV-BQ1 is a member of the proposed order Megavirales with characteristics of both phycodnaviruses and mimiviruses indicating that, in addition to its complex ecological dynamics, it also has a complex evolutionary history. |
| Databáze: | OpenAIRE |
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