A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate
| Autor: | Divya Mamilla, Bhavana Bhaya, Theresa Czech, Esther Kisseih, Nitin Chouthai, Arun Saini, Rahul Rajkumar |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Enzyme-Linked Immunosorbent Assay Gestational Age Umbilical cord Andrology 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Developmental Neuroscience Pregnancy Glial cell line-derived neurotrophic factor medicine Humans Glial Cell Line-Derived Neurotrophic Factor biology urogenital system business.industry Age Factors Infant Newborn Gestational age Aging Premature medicine.disease Fetal Blood Glial cell derived neurotrophic factor 030104 developmental biology medicine.anatomical_structure nervous system Anesthesia Cord blood biology.protein Gestation Female business 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 65 |
| ISSN: | 1873-474X |
| Popis: | Background This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns’ umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions. Methods Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background. Results Cord blood GDNF levels were significantly higher (p = 0.004) in preterm newborns (n = 6) (115.05 ± 57.17, pg/ml) when compared to term newborns (n = 14) (19.67 ± 10.67, pg/ml). GDNF levels in CSF trended (p = 0.07) higher in term newborns (n = 10) (19.56 ± 9.11, pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n = 5) (14.49 ± 3.53, pg/ml). Conclusions GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions. |
| Databáze: | OpenAIRE |
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