Identification and characterization of proteins that are differentially expressed in adipose tissue of olanzapine-induced insulin resistance rat by iTRAQ quantitative proteomics
| Autor: | Lixiu Yu, Jing Wang, Ni Yang, Qiangqiang Han, Yahui Deng, Zhongfang Zhai, Liangyu Yu, Weiyong Li |
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| Rok vydání: | 2020 |
| Předmět: |
Proteomics
0301 basic medicine Quantitative proteomics Biophysics Adipose tissue Computational biology Fatty Acid-Binding Proteins Biochemistry Cell Line Rats Sprague-Dawley 03 medical and health sciences Insulin resistance Western blot Tandem Mass Spectrometry Glucose Intolerance medicine Animals Apolipoproteins A 030102 biochemistry & molecular biology medicine.diagnostic_test Mechanism (biology) Chemistry Akt/PKB signaling pathway Computational Biology medicine.disease Rats 030104 developmental biology Adipose Tissue Gene Expression Regulation Olanzapine YWHAZ Female Insulin Resistance Signal transduction Biomarkers Antipsychotic Agents Signal Transduction |
| Zdroj: | Journal of Proteomics. 212:103570 |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2019.103570 |
| Popis: | Olanzapine is commonly used to treat schizophrenia. However, long-term administration of olanzapine causes metabolic side effects, such as insulin resistance (IR), which seriously affects patients' quality of life. Both diagnostic and prognostic markers are urgently needed to increase patient compliance. We applied isobaric tags for relative and absolute quantitation (iTRAQ) labeling combined with 2D LC/MS/MS technology to identify the differentially expressed proteins in olanzapine-induced IR rats. A total of 3194 proteins were identified from rat adipose tissues, and 270 differentially expressed proteins were screened out with a ratio threshold >1.5-fold or |
| Databáze: | OpenAIRE |
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