Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells
| Autor: | Phillip A. Sharp, Mark Williams, J. P. Tennant, S. Yamaji, S Tandy, Surjit K. S. Srai |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Endosome Cations Divalent Iron Biophysics Down-Regulation Gene Expression Heme Biochemistry Caco-2 cell Cell membrane Structural Biology Iron-Binding Proteins Receptors Transferrin Genetics medicine Humans RNA Messenger Iron transport Molecular Biology Cation Transport Proteins chemistry.chemical_classification biology Dose-Response Relationship Drug digestive oral and skin physiology Microfilament Proteins Transporter Cell Differentiation Cell Biology DMT1 Apical membrane Intestines Cytosol medicine.anatomical_structure chemistry Caco-2 Transferrin Metals Divalent metal transporter biology.protein Caco-2 Cells Carrier Proteins |
| Zdroj: | FEBS letters. 510(1-2) |
| ISSN: | 0014-5793 |
| Popis: | A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non-haem iron. Using human intestinal Caco-2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration-dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron. |
| Databáze: | OpenAIRE |
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