Identification of a bacteriophage from an environmental multidrug-resistant E. coli isolate and its function in horizontal transfer of ARGs
| Autor: | Qingxiang Yang, Xiangpeng Zeng, Qiang Wang, Chuanzhen Yang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Environmental Engineering Gene Transfer Horizontal 030106 microbiology Wastewater medicine.disease_cause Genome Bacteriophage 03 medical and health sciences chemistry.chemical_compound Transduction (genetics) Drug Resistance Bacterial medicine Escherichia coli Environmental Chemistry Bacteriophages Waste Management and Disposal Genetics biology Bacteria biology.organism_classification Pollution Anti-Bacterial Agents Multiple drug resistance chemistry Genes Bacterial Horizontal gene transfer DNA |
| Zdroj: | The Science of the total environment. 639 |
| ISSN: | 1879-1026 |
| Popis: | Horizontal transfer of ARGs was generally considered to be mediated by three methods – transformation, conjugation and transduction through phages – during which the contribution of bacteriophages to gene transfer in the environment is unclear or even questioned. In this study, a multiple-antibiotic-resistant Escherichia coli strain and its phage (YZ1) were isolated from a municipal wastewater treatment system. The results of the morphological and genomic analyses of phage YZ1 showed that it is a member of the T7 viral genus in the subfamily Autographivirinae. Its genome is similar to that of the E. coli phage K1F in both organization and sequence and does not encode ARGs. However, 28 paired reads in the raw sequencing data aligned to ARGs, including those promoting β-lactam, aminoglycoside, and fluoroquinolone resistance, among others. Quantitative PCR showed that ARGs were present in bacteriophage DNA (approximately 103 copies/mL) and were also detected in the bacterial host DNA. The results suggested that while infrequent, some ARG-carrying transducing phages were presumably generated by erroneous packaging during infection of antibiotic-resistant bacteria, which may create the possibility of horizontal transfer of ARGs. |
| Databáze: | OpenAIRE |
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