Inhibition of Herpesvirus-6B RNA Replication by Short Interference RNAs
| Autor: | Yong-Tae Jung, Min-Chul Shin, In-Gu Khang, Soon-Young Paik, Wan-Shik Shin, Chun-Choo Kim, Sun-Hwa Kim, Dong-Gun Lee, Jong-Sub Yoon, Seong-Karp Hong |
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| Rok vydání: | 2004 |
| Předmět: |
Small interfering RNA
DNA polymerase Herpesvirus 6 Human T-Lymphocytes viruses RNA-dependent RNA polymerase Virus Replication Biochemistry RNA interference DNA-directed RNA interference Animals Humans Gene silencing RNA Small Interfering Molecular Biology Cells Cultured RNA Double-Stranded Base Sequence biology fungi virus diseases RNA General Medicine Virology RNA silencing biology.protein RNA Viral |
| Zdroj: | BMB Reports. 37:383-385 |
| ISSN: | 1976-6696 |
| DOI: | 10.5483/bmbrep.2004.37.3.383 |
| Popis: | RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication. |
| Databáze: | OpenAIRE |
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