Partially Penetrant Postnatal Lethality of an Epithelial Specific MicroRNA in a Mouse Knockout
| Autor: | Chong Yon Park, D’Juan T. Farmer, Nikki Shariat, Michael T. McManus, Anastasia Mavropoulos, Huey Jiin Liu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
lcsh:Medicine
Penetrance In situ hybridization Biology Epithelium Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo microRNA Gene expression medicine Animals Beta-galactosidase lcsh:Science In Situ Hybridization 030304 developmental biology Mice Knockout Genetics 0303 health sciences Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction lcsh:R Gene Expression Regulation Developmental Embryo Embryo Mammalian beta-Galactosidase Survival Analysis 3. Good health Cell biology MicroRNAs medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis biology.protein Genes Lethal lcsh:Q Penetrant (biochemical) Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e76634 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | MicroRNAs are small noncoding RNAs thought to have pivotal roles in numerous diseases and developmental processes. However, a growing body of literature indicates that in vivo elimination of these tiny RNAs usually has little to no observable consequence, suggesting functional redundancy with other microRNAs or cellular pathways. We provide an in-depth analysis of miR-205 expression and define miR-205 as an epithelial-specific microRNA, and for the first time show that ablation of this microRNA knockout exhibits partially penetrant lethality in a constitutive mouse knockout model. Given the role of this microRNA in cancer and development, this mouse model will be an incredible reagent to study the function and mechanisms of miR-205 in epithelial tissue development and disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|