CCHF virus variants in Pakistan and Afghanistan: Emerging diversity and epidemiology
| Autor: | Mukhtiar Hassan, Muhammad Masroor Alam, Lubna Rehman, Syed Sohail Zahoor Zaidi, Muhammad Suleman Rana, Uzma Bashir Aamir, Mehar Angez, Salmaan Sharif, S. Shahid Shaukat, Adnan Khurshid |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Crimean–Congo hemorrhagic fever medicine.medical_specialty Adolescent Endemic Diseases Sequence Homology Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Young Adult Virology Epidemiology medicine Cluster Analysis Humans Pakistan Viral rna CCHF VIRUS Phylogeny Molecular Epidemiology Genetic diversity Middle East Molecular epidemiology Traditional medicine Reverse Transcriptase Polymerase Chain Reaction Mortality rate Afghanistan Genetic Variation Sequence Analysis DNA Middle Aged medicine.disease Infectious Diseases Immunoglobulin M Hemorrhagic Fever Virus Crimean-Congo RNA Viral Female Hemorrhagic Fever Crimean |
| Zdroj: | Journal of Clinical Virology. 67:25-30 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2015.03.021 |
| Popis: | Background Crimean Congo hemorrhagic fever (CCHF) has been reported from more than 30 countries in Africa, Asia, Eastern Europe and Middle East. The disease is considered endemic in Pakistan and neighboring countries like Iran and Afghanistan. Objectives This study aimed to explore the genetic diversity of CCHF virus (CCHFV) detected in Pakistan and Afghanistan based on analysis of partial S-segment sequences. Study design During 2011, one hundred samples satisfying the CCHF case definition were tested by (ELISA) and RT-PCR for detection of IgM antibodies and viral RNA, respectively. Phylogenetic analysis was carried out on partial S-segment nucleotide sequences using MEGA 5.0. Results Out of one hundred collected during 2011, 49 (49%) were positive for CCHF either by ELISA/RT-PCR or both. The mean age of the CCHFV positive cases was 30.32 years (range 18–56 years) and overall mortality rate was 20.4%. All CCHF virus isolates from this study clustered with strains previously reported from Pakistan, Iran and Afghanistan within the Asia-1 genogroup. Four distinct sub-clades were found circulating within Asia-1 genogroup. Six CCHFV strains found in Pakistan and Afghanistan grouped into a new sub-clade-D. Conclusions Data from this study shows that endemic foci of CCHFV span the international border between Pakistan and Afghanistan with genetically diverse variants circulating in this region. Our findings emphasize to establish a laboratory based surveillance program and devise health policy measures to control CCHF infection especially in Baluchistan. |
| Databáze: | OpenAIRE |
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