Analysis of home dust for Staphylococcus aureus and staphylococcal enterotoxin genes using quantitative PCR
| Autor: | Elizabeth C. Matsui, Sonali Bose, Shanna Ludwig, Gregory B. Diette, Meredith C. McCormack, Isabel Jimenez-Bush, Meghan F. Davis, Emily P. Brigham |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Allergy Environmental Engineering 030106 microbiology Enterotoxin 010501 environmental sciences Biology medicine.disease_cause 01 natural sciences Article Microbiology Enterotoxins 03 medical and health sciences Enterotoxin gene medicine Superantigen Environmental Chemistry Waste Management and Disposal DNA Primers 0105 earth and related environmental sciences Asthma Dust medicine.disease biology.organism_classification Pollution respiratory tract diseases Real-time polymerase chain reaction Genes Bacterial Immunology Housing Bacteria |
| Zdroj: | Science of The Total Environment. :750-755 |
| ISSN: | 0048-9697 |
| Popis: | The bacterium Staphylococcus aureus (SA) is known to induce allergic inflammatory responses, including through secreted staphylococcal enterotoxin (SE) superantigens. To quantify indoor environmental exposures to these potential allergens, which may be associated with worse asthma, we developed a method for the assessment of S. aureus and SE in home dust and applied it to a study of homes of inner-city adults with asthma.We conducted laboratory experiments to optimize sample processing and real-time PCR methods for detection and quantification of SA (femB) and SEA-D, based on published primers. We applied this method to dust and dust extract from 24 homes. We compared results from real-time PCR to culture-based results from the same homes.The bacteremia DNA isolation method provided higher DNA yield than alternative kits. Culture-based results from homes demonstrated 12 of 24 (50%) bedrooms were contaminated with S. aureus, only one of which carried a SE gene (SEC). In contrast, femB was detected in 23 of 24 (96%) bedrooms with a median of 1.1×10Our culture-independent method to detect S. aureus and SE in home dust was more sensitive than our culture-based method. Prevalence of household exposure to S. aureus and SE allergens may be high among adults with asthma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect