Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation
| Autor: | Shunro Kai, Tamami Kobayashi, Satoshi Yoshihara, Junko Ikemoto, Yoshihiro Fujimori |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research CD34 Antigens CD34 Cord Blood Stem Cell Transplantation Biology Biochemistry Gene Expression Regulation Enzymologic Andrology Colony-Forming Units Assay 03 medical and health sciences 0302 clinical medicine Genetics Humans Cell Lineage Molecular Biology Cryopreservation Cluster of differentiation Umbilical Cord Blood Transplantation Macrophages Stem Cells Cell Differentiation Aldehyde Dehydrogenase Fetal Blood Flow Cytometry Hematopoietic Stem Cells Transplantation Haematopoiesis 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cord blood Molecular Medicine Stem cell Granulocytes |
| Zdroj: | Molecular medicine reports. 18(5) |
| ISSN: | 1791-3004 |
| Popis: | In umbilical cord blood transplantation (UCBT), the number of cluster of differentiation (CD)34+ cells and colony‑forming units (CFUs) in the cord blood (CB) graft positively correlate with patient survival. Therefore, these parameters are currently used for quality assessment of the cryopreserved CB cells in the attached segment that is considered representative of the CB in the main bag prior to UCBT. Since aldehyde dehydrogenase (ALDH) activity is high in hematopoietic stem cells, the number of ALDH‑bright (ALDHbr) cells was examined in comparison with the number of CD34+ cells and CFUs for the quality assessment of CB units. In the cryopreserved main bag, the number of ALDHbr cells in the CB unit exhibited positive correlation with the number of CD34+ cells, and with CFU‑granulocytes/macrophages and total CFU counts. Furthermore, the concentration of ALDHbr cells in the cryopreserved attached segment was not significantly different compared to that of the main bag, suggesting that the attached segment is representative of the main bag. In conclusion, the present study suggested that ALDHbr cell counts in the cryopreserved attached segments may serve as a quality assessment indicator for CB units prior to UCBT. |
| Databáze: | OpenAIRE |
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