A Randomized Placebo Controlled Trial of Ibuprofen for Respiratory Syncytial Virus Infection in a Bovine Model
| Autor: | Mark L Anderson, Paul Walsh, Heather A. McEligot, John W. Newman, Nicole E. Behrens, Laurel J. Gershwin, Karan Agrawal, Francisco R. Carvallo Chaigneau |
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| Přispěvatelé: | Tregoning, John S |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pulmonology NSAIDs Physiology viruses Placebo-controlled study Anti-Inflammatory Agents lcsh:Medicine Respiratory Syncytial Virus Bovine Ibuprofen Biochemistry Immunoenzyme Techniques 0302 clinical medicine Immune Physiology Medicine and Health Sciences Respiratory system lcsh:Science Lung 2. Zero hunger Pediatric Analgesics Innate Immune System Multidisciplinary Anti-Inflammatory Agents Non-Steroidal Drugs Bovine respiratory system Viral Load Lipids 3. Good health medicine.anatomical_structure Infectious Diseases Bronchiolitis Cytokines Anatomy Infection Non-Steroidal Viral load Research Article Viral Release Histology General Science & Technology Immunology Clinical Trials and Supportive Activities Cattle Diseases Respiratory Syncytial Virus Infections Biology Microbiology Virus Lymphatic System 03 medical and health sciences Lipid Mediators Clinical Research Virology medicine Animals Adverse effect Pharmacology Animal organic chemicals lcsh:R Biology and Life Sciences Molecular Development medicine.disease Pain management Disease Models Animal 030104 developmental biology Immune System Respiratory Infections Disease Models biology.protein lcsh:Q Cattle Cyclooxygenase Lymph Nodes Respiratory Syncytial Virus 030217 neurology & neurosurgery Viral Transmission and Infection Developmental Biology |
| Zdroj: | PloS one, vol 11, iss 4 PLoS ONE, Vol 11, Iss 4, p e0152913 (2016) PLoS ONE |
| Popis: | Background Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and hospital admission in infants. An analogous disease occurs in cattle and costs US agriculture a billion dollars a year. RSV causes much of its morbidity indirectly via adverse effects of the host response to the virus. RSV is accompanied by elevated prostaglandin E2 (PGE2) which is followed by neutrophil led inflammation in the lung. Ibuprofen is a prototypical non-steroidal anti-inflammatory drug that decreases PGE2 levels by inhibiting cyclooxygenase. Hypotheses We hypothesized that treatment of RSV with ibuprofen would decrease PGE2 levels, modulate the immune response, decrease clinical illness, and decrease the histopathological lung changes in a bovine model of RSV. We further hypothesized that viral replication would be unaffected. Methods We performed a randomized placebo controlled trial of ibuprofen in 16 outbred Holstein calves that we infected with RSV. We measured clinical scores, cyclooxygenase, lipoxygenase and endocannabinoid products in plasma and mediastinal lymph nodes and interleukin (Il)-4, Il-13, Il-17 and interferon-γ in mediastinal lymph nodes. RSV shedding was measured daily and nasal Il-6, Il-8 and Il-17 every other day. The calves were necropsied on Day 10 post inoculation and histology performed. Results One calf in the ibuprofen group required euthanasia on Day 8 of infection for respiratory distress. Clinical scores (p |
| Databáze: | OpenAIRE |
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