Diversity and antibacterial activity of fungal endophytes from Eucalyptus exserta
| Autor: | Yuanhang Peng, Zining Cui, Chunyin Wu, Ping Ding, Ziling Mao, Tijiang Shan, Hao Feng, Hamza Shahid, Weihao Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Microbiology (medical) Spectrometry Mass Electrospray Ionization Phyllosticta Talaromyces Endophytic fungi 01 natural sciences Microbiology Plant use of endophytic fungi in defense Botany Endophytes Pestalotiopsis Eucalyptus Ralstonia solanacearum Plant Stems biology 010405 organic chemistry Eutypella Research Secondary metabolites Eucalyptus exserta Fungi food and beverages Chaetomium biology.organism_classification QR1-502 Anti-Bacterial Agents 0104 chemical sciences Fruit Antibacterial activity Lophiostoma sp. Eef-7 010606 plant biology & botany Cladosporium |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 21, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-021-02229-8 |
| Popis: | Background Eucalyptus bacterial wilt caused by Ralstonia solanacearum is an important eucalyptus disease. Endophytic fungi, an important source of natural active substances, provide a new breakthrough for the control of plant diseases. Results In the present study, 80 endophytic fungal isolates were obtained from the healthy branches and fruits of Eucalyptus exserta. Fifteen distinct isolates (MK120854-MK120868) were selected for further taxonomic identification through morphological trait assessments and internal transcribed spacer (ITS) region-rRNA gene sequence analysis. Thirteen genera, namely, Phyllosticta, Penicillium, Eutypella, Purpureocillium, Talaromyces, Lophiostoma, Cladosporium, Pestalotiopsis, Chaetomium, Fusarium, Gongronella, Scedosporium and Pseudallescheria, were identified on the basis of their morphological characteristics. Members of the genus Phyllosticta were the primary isolates, with a colonization frequency (CF) of 27.5 %. Most of the fungal isolates displayed antibacterial activity. The crude extracts obtained from Lophiostoma sp. Eef-7, Pestalotiopsis sp. Eef-9 and Chaetomium sp. Eef-10 exhibited strong inhibition on the test bacteria, and Lophiostoma sp. Eef-7 was further cultured on a large scale. Three known compounds, scorpinone (1), 5-deoxybostrycoidin (2) and 4-methyl-5,6-dihydro-2 H-pyran-2-one (3), were isolated from the endophytic fungus Lophiostoma sp. Eef-7 associated with E. exserta. The structures of these compounds were elucidated by analysis of 1D and 2D NMR and HR-ESI-MS spectra and a comparison of their spectral data with published values. Compounds 1 and 2 showed weak antimicrobial activity against Ralstonia solanacearum. Conclusions Endophytic fungi from Eucalyptus exserta may represent alternative sources of antimicrobial agents. Lophiostoma sp. Eef-7 can produce 2-azaanthraquinone derivatives and shows weak antibacterial activity against Ralstonia solanacearum. |
| Databáze: | OpenAIRE |
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