Auxin efflux carriers, MiPINs, are involved in adventitious root formation of mango cotyledon segments
| Autor: | Yun-He Li, Yi-Wei Mo, Song-Biao Wang, Zhi Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Auxin efflux Auxin influx Physiology Arabidopsis Endogeny Plant Science 01 natural sciences Plant Roots 03 medical and health sciences chemistry.chemical_compound Biosynthesis Plant Growth Regulators Triiodobenzoic Acids Genetics Vascular tissue Plant Proteins Mangifera biology Indoleacetic Acids Chemistry food and beverages Membrane Transport Proteins Embryo biology.organism_classification Plants Genetically Modified Cell biology 030104 developmental biology Polar auxin transport Cotyledon 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry : PPB. 150 |
| ISSN: | 1873-2690 |
| Popis: | Adventitious roots form only at the proximal cut surface (PCS) but not at the distal cut surface (DCS) of mango cotyledon segments. In this study, mango embryos treated with indole-3-butyric acid (IBA) showed significantly increased adventitious root formation, while those treated with 2, 3, 5-triiodobenzoic acid (TIBA) demonstrated complete inhibition of adventitious rooting. Mango embryos treated with auxin influx inhibitors demonstrated lower inhibition of adventitious roots than those treated with TIBA. The endogenous indol-3-acetic acid (IAA) content on the PCS and DCS was similar at 0 h, then increased on both surfaces after 6 h, and IAA content on the PCS were always higher than those on the DCS. We cloned three genes encoding auxin efflux carriers (i.e., MiPIN2-4) and examined their temporal and spatial expression patterns under different treatments. Relative expression of all MiPINs studied was very low at 0 h but significantly increased on both PCS and DCS from 1 d to 10 d, to varying degrees. We overexpressed MiPIN1-4 in Arabidopsis plants and found a significant increase in adventitious root quantity in MiPIN1 and MiPIN3 transgenic lines. Immunofluorescence results showed that MiPIN1 and MiPIN3 are primarily localized in the vascular tissues and the cells adjacent to abaxial surface. In conclusion, we propose that in mango cotyledon segments, wounding stimulates IAA biosynthesis, the transcription levels of PIN genes were significantly increased in different magnitudes on the PCS and DCS, resulting in polar IAA transport from the DCS to PCS via the vascular tissues, thereby triggering adventitious root formation. |
| Databáze: | OpenAIRE |
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