Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
| Autor: | I. Saira Mian, Trevor Eydmann, Teresa S. Wang, Takabumi Inagawa, Jacob Z. Dalgaard, Tomoko Yamada-Inagawa |
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| Rok vydání: | 2009 |
| Předmět: |
DNA Replication
Amino Acid Motifs Molecular Sequence Data Pre-replication complex DNA replication factor CDT1 Replication factor C Minichromosome maintenance Control of chromosome duplication Proliferating Cell Nuclear Antigen Two-Hybrid System Techniques Schizosaccharomyces Amino Acid Sequence Genetics Multidisciplinary biology Sequence Homology Amino Acid Ter protein Biological Sciences DNA-Binding Proteins Licensing factor Phenotype Gene Expression Regulation Mutation biology.protein Small Ubiquitin-Related Modifier Proteins Origin recognition complex Schizosaccharomyces pombe Proteins Genome Fungal Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 106(19) |
| ISSN: | 1091-6490 |
| Popis: | Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1 . We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of “slow-moving” Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2 -dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin. |
| Databáze: | OpenAIRE |
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