Biomechanics of the optic nerve head and peripapillary sclera in a mouse model of glaucoma
| Autor: | Thao D. Nguyen, Elizabeth C. Kimball, Harry A. Quigley, Joan L. Jefferys, Arina Korneva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Lamina genetic structures education Optic Disk Biomedical Engineering Biophysics Ocular hypertension Glaucoma Bioengineering Strain (injury) Biochemistry Biomaterials 03 medical and health sciences Mice 0302 clinical medicine Ophthalmology medicine Circumferential strain Animals health care economics and organizations Intraocular Pressure Life Sciences–Engineering interface 030304 developmental biology 0303 health sciences business.industry Biomechanics medicine.disease eye diseases Sclera Biomechanical Phenomena medicine.anatomical_structure 030221 ophthalmology & optometry Optic nerve sense organs business Biotechnology |
| Zdroj: | J R Soc Interface |
| ISSN: | 1742-5662 |
| Popis: | The deformation of the mouse astrocytic lamina (AL) and adjacent peripapillary sclera (PPS) was measured in response to elevated intraocular pressure. We subjected explanted mouse eyes to inflation testing, comparing control eyes to those 3 days and 6 weeks after induction of ocular hypertension (OHT) via ocular microbead injection. Laser scanning microscopy was used with second harmonic generation to image the collagenous PPS and two-photon fluorescence to image transgenic fluorescent astrocytes in the AL. Digital volume correlation was applied to calculate strains in the PPS and AL. The specimen-averaged strains were biaxial in the AL and PPS, with greater strain overall in the x- than y- direction in the AL and greater strain in the θ- than the r -direction in the PPS. Strains increased after 3-day OHT, with greater strain overall in the 3-day AL than control AL, and greater circumferential strain in the 3-day PPS than control PPS. In the 6-week OHT eyes, AL and PPS strains were similar overall to controls. This experimental glaucoma model demonstrated a dynamic change in the mechanical behaviour of the AL and PPS over time at the site of neuronal injury and remodelling in glaucoma. |
| Databáze: | OpenAIRE |
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