Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X
| Autor: | Kyoung-Min Jo, Hyosub Chu, Sen Lian, Ju-Yeon Yoon, Kook-Hyung Kim, Hoseong Choi, Yeonhwa Jo, Won Kyong Cho, Seung-Kook Choi |
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| Rok vydání: | 2014 |
| Předmět: |
Expressed sequence tag
Chloroplasts biology Chrysanthemum RNA Nucleic Acid Hybridization Plant Science General Medicine Potato virus X biology.organism_classification Real-Time Polymerase Chain Reaction Virology Virus Plant Viruses Transcriptome Cucumber mosaic virus Plant virus Genetics RNA Viruses Agronomy and Crop Science Gene Oligonucleotide Array Sequence Analysis Transcription Factors |
| Zdroj: | Plant molecular biology. 88(3) |
| ISSN: | 1573-5028 |
| Popis: | The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses. |
| Databáze: | OpenAIRE |
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