Simultaneous quantification of ten key Kratom alkaloids in Mitragyna speciosa leaf extracts and commercial products by ultra‐performance liquid chromatography−tandem mass spectrometry
| Autor: | Francisco León, Tamara I. King, Christopher R. McCurdy, Nelson Jeng Yeou Chear, Bonnie A. Avery, Surash Ramanathan, Abhisheak Sharma, Shyam H. Kamble, Erin C. Berthold |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Mitragyna speciosa
Pharmaceutical Science Mass spectrometry 01 natural sciences Article Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Environmental Chemistry 030216 legal & forensic medicine Chromatography High Pressure Liquid Spectroscopy Mitraphylline Chromatography biology Mitragyna Plant Extracts Alkaloid 010401 analytical chemistry biology.organism_classification 0104 chemical sciences Plant Leaves chemistry Mitragynine 7-Hydroxymitragynine Ammonium acetate |
| Zdroj: | Drug Test Anal |
| ISSN: | 1942-7611 1942-7603 |
| Popis: | Kratom (Mitragyna speciosa) is a psychoactive plant popular in the United States for the self-treatment of pain and opioid addiction. For standardization and quality control of raw and commercial kratom products, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of ten key alkaloids, namely: corynantheidine, corynoxine, corynoxine B, 7-hydroxymitragynine, isocorynantheidine, mitragynine, mitraphylline, paynantheine, speciociliatine, and speciogynine. Chromatographic separation of diastereomers, or alkaloids sharing same ion transitions, was achieved on an Acquity BEH C18 column with a gradient elution using a mobile phase containing acetonitrile and aqueous ammonium acetate buffer (10mM, pH 3.5). The developed method was linear over a concentration range of 1-200 ng/mL for each alkaloid. The total analysis time per sample was 22.5 minutes. The analytical method was validated for accuracy, precision, robustness, and stability. After successful validation, the method was applied for the quantification of kratom alkaloids in alkaloid-rich fractions, ethanolic extracts, lyophilized teas, and commercial products. Mitragynine (0.7%-38.7% w/w), paynantheine (0.3%-12.8% w/w), speciociliatine (0.4%-12.3% w/w), and speciogynine (0.1%-5.3% w/w) were the major alkaloids in the analyzed kratom products/extracts. Minor kratom alkaloids (corynantheidine, corynoxine, corynoxine B, 7-hydroxymitragynine, isocorynantheidine) were also quantified (0.01%-2.8% w/w) in the analyzed products; however mitraphylline was below the lower limit of quantification in all analyses. |
| Databáze: | OpenAIRE |
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