In vitro 3D Systems to Model Tumor Angiogenesis and Interactions With Stromal Cells
| Autor: | Stéphane Germain, Laurent Muller, Noémie Brassard-Jollive, Catherine Monnot |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
3D micro-tumors Stromal cell Angiogenesis microfluidic Review Extracellular matrix Cell and Developmental Biology 03 medical and health sciences angiogenesis 0302 clinical medicine Vasculogenesis tumor microenvironment tumor–stromal cell interactions lcsh:QH301-705.5 Sprouting angiogenesis Tumor microenvironment Chemistry Intravasation Cell Biology personalized medicine Cell biology 030104 developmental biology lcsh:Biology (General) Tumor progression 030220 oncology & carcinogenesis Developmental Biology |
| Zdroj: | Frontiers in Cell and Developmental Biology, Vol 8 (2020) Frontiers in Cell and Developmental Biology |
| DOI: | 10.3389/fcell.2020.594903/full |
| Popis: | In vitro 3D culture systems provide promising tools for screening novel therapies and understanding drug resistance mechanisms in cancer because they are adapted for high throughput analysis. One of the main current challenges is to reproducibly culture patient samples containing cancer and stromal cells to faithfully recapitulate tumor microenvironment and move toward efficient personalized medicine. Tumors are composed of heterogeneous cell populations and characterized by chaotic vascularization in a remodeled microenvironment. Indeed, tumor angiogenesis occurs in a complex stroma containing immune cells and cancer-associated fibroblasts that secrete important amounts of cytokines, growth factors, extracellular vesicles, and extracellular matrix (ECM). This process leads to the formation of inflated, tortuous, and permeable capillaries that display deficient basement membrane (BM) and perivascular coverage. These abnormal capillaries affect responses to anti-cancer therapies such as anti-angiogenic, radio-, and immunotherapies. Current pre-clinical models are limited for investigating interactions between tumor cells and vascularization during tumor progression as well as mechanisms that lead to drug resistance. In vitro approaches developed for vascularization are either the result of engineered cell lining or based on physiological processes including vasculogenesis and sprouting angiogenesis. They allow investigation of paracrine and direct interactions between endothelial and tumor and/or stromal cells, as well as impact of biochemical and biophysical cues of the microenvironment, using either natural matrix components or functionalized synthetic hydrogels. In addition, microfluidic devices provide access to modeling the impact of shear stress and interstitial flow and growth factor gradients. In this review, we will describe the state of the art co-culture models of vascularized micro-tumors in order to study tumor progression and metastatic dissemination including intravasation and/or extravasation processes. |
| Databáze: | OpenAIRE |
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