Determination of platinum originated from antitumoral drugs in human urine by atomic absorption spectrometric methods
| Autor: | Mariana Antunes Vieira, Anilton Coelho da Costa, Aderval S. Luna, Reinaldo Calixto de Campos |
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| Rok vydání: | 2010 |
| Předmět: |
Detection limit
Analyte Central composite design Spectrophotometry Atomic Analytical chemistry Reproducibility of Results chemistry.chemical_element Antineoplastic Agents Platinum Compounds In Vitro Techniques Carboplatin Analytical Chemistry law.invention Matrix (chemical analysis) chemistry.chemical_compound chemistry Limit of Detection law Calibration Humans Cisplatin Atomic absorption spectroscopy Platinum |
| Zdroj: | Talanta. 82:1647-1653 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2010.07.029 |
| Popis: | Cisplatin and carboplatin are the most common platinum-based drugs used in cancer treatment. Pharmacokinetic investigations, the evaluation of the body burden during the treatment, as well as baseline levels of platinum in humans have attracted great interest. Thus, accurate analytical methods for fast and easy Pt monitoring in clinical samples become necessary. In the present study atomic absorption spectrometric methods for the determination of platinum in the forms of cisplatin and carboplatin in human urine were investigated. Platinum, in these different forms, could be determined in urine, after simple sample dilution. Regarding electrothermal atomic absorption spectrometry, the optimum parameters were defined by a central composite design optimization. Multiplicative matrix effects were overcome by using a mixture of HCl and NaCl as modifier. The limit of detection (LOD) was 0.004 mg L−1 of platinum in the original sample. For the analysis of more concentrated samples, high resolution continuous source flame atomic absorption spectrometry was also investigated. Flame conditions were optimized by a multivariate D-optimal design, using as response the sum of the analyte addition calibration slopes and their standard deviations. Matrix matched external calibration with PtCl2 calibration solutions, was possible, and the LOD was 0.06 mg L−1 in the original sample. The results obtained by the proposed procedures were also in good agreement with those obtained by an independent comparative procedure. |
| Databáze: | OpenAIRE |
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