Evaluation of BioFM liquid medium for culture of cerebrospinal fluid in tuberculous meningitis to identify Mycobacterium tuberculosis
| Autor: | Hatim F. Daginawala, Girdhar M. Taori, Hemant J. Purohit, Hari M. Gaherwar, Rajpal S. Kashyap, Sonali S Ramteke, Poonam S. Deshpande |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Adult Male Pathology medicine.medical_specialty Tuberculosis Adolescent Liquid culture Liquid medium urologic and male genital diseases Sensitivity and Specificity Tuberculous meningitis Microbiology Mycobacterium tuberculosis Young Adult Cerebrospinal fluid medicine Humans Aged Cerebrospinal Fluid Bacteriological Techniques biology business.industry Middle Aged medicine.disease biology.organism_classification Culture Media Tuberculosis Meningeal Female business Meningitis |
| Zdroj: | Indian journal of medical microbiology. 28(4) |
| ISSN: | 1998-3646 |
| Popis: | The present study was designed to evaluate the sensitivity and specificity of liquid culture medium (BioFM broth) for the diagnosis of tuberculous meningitis (TBM) in cerebrospinal fluid (CSF). CSF samples from 200 patients (TBM group = 150 and non-TBM group = 50) were tested for culture of Mycobacterium tuberculosis in BioFM liquid culture medium. Out of 150 TBM cases, 120 were found to be culture positive, indicating a sensitivity of 80% in BioFM broth within 2-3 weeks of inoculation. Positive cultures were also observed for CSF from 32 (64%) out of 50 non-TBM patients in BioFM liquid culture medium within 4 days of sample inoculation. Therefore, according to our study, BioFM broth system yielded 80% sensitivity [95% confidence interval (CI): 67-93%] and 36% specificity (95% CI: 57-98%) for TBM diagnosis. Our results indicate that although BioFM broth allows the detection of positive cultures within a shorter time, it has a high potential for contamination or for the coexistence of M. tuberculosis and non-tuberculous meningitis (NTM). This coexistence may go undetected or potentially lead to erroneous reporting of results. |
| Databáze: | OpenAIRE |
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