Expression Profiling ofYersinia pestisDuring Mouse Pulmonary Infection
| Autor: | Jonathan N. Lawson, C. Rick Lyons, Stephen Albert Johnston |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Flea
Genomic Islands Transcription Genetic Yersinia pestis Colony Count Microbial Biology Bubonic plague Microbiology Pathogenesis Mice In vivo Genetics medicine Animals Lung Molecular Biology Pathogen Administration Intranasal Oligonucleotide Array Sequence Analysis Base Composition Mice Inbred BALB C Plague Plague Vaccine Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Temperature Cell Biology General Medicine biology.organism_classification medicine.disease Bioterrorism Virology In vitro Gene expression profiling Liver Cats RNA Spleen |
| Zdroj: | DNA and Cell Biology. 25:608-616 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2006.25.608 |
| Popis: | Yersinia pestis, the causative agent of plague, can be transmitted by infected flea bite or inhaled aerosol. Both routes of infection have a high mortality rate, and pneumonic infections of Y. pestis represent a significant concern as a tool of bioterrorism. Understanding the transcriptional program of this pathogen during pulmonary infection should be valuable in understanding plague pathogenesis, as well as potentially offering insights into new vaccines and therapeutics. Toward this goal we developed a long oligonucleotide microarray to the plague bacillus and evaluated the expression profiles of Y. pestis in vitro and in the mouse pulmonary infection model in vivo. The in vitro analysis compared expression patterns at 27 versus 37 degrees C, as a surrogate of the transition from the flea to the mammalian host. The in vivo analysis used intranasal challenge to the mouse lung. By amplifying the Y. pestis RNA from individual mouse lungs we were able to map the transcriptional profile of plague at postinfection days 1 to 3. Our data present a very different transcriptional profile between in vivo and in vitro expression, suggesting Y. pestis responds to a variety of host signals during infection. Of note was the number of genes found in genomic regions with altered %GC content that are upregulated within the mouse lung environment. These data suggest these regions may provide particularly promising targets for both vaccines and therapeutics. |
| Databáze: | OpenAIRE |
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