Targeted delivery of CpG ODN to CD32 on human and monkey plasmacytoid dendritic cells augments IFNalpha secretion
| Autor: | B. A. ‘t Hart, Gerrit Koopman, Geert C. Mudde, Niels Beenhakker, I. Jolanda M. de Vries, Jurjen Tel |
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| Rok vydání: | 2012 |
| Předmět: |
CD32
CpG Oligodeoxynucleotide Immunology Plasmacytoid dendritic cell Receptors Fc Proinflammatory cytokine Immune Regulation [NCMLS 2] Immunology and Allergy Animals Humans Secretion Biotinylation Cells Cultured CD86 biology Receptors IgG Translational research Immune Regulation [ONCOL 3] TLR9 Interferon-alpha hemic and immune systems Hematology Dendritic Cells Macaca mulatta Oligodeoxyribonucleotides Toll-Like Receptor 9 biology.protein Cytokines Inflammation Mediators CD80 |
| Zdroj: | Immunobiology, 217, 10, pp. 1017-24 Immunobiology, 217, 1017-24 |
| ISSN: | 0171-2985 |
| Popis: | Contains fulltext : 109682.pdf (Publisher’s version ) (Open Access) Atopic diseases are characterized by the presence of Th2 cells. Recent studies, in mice and man, demonstrated that allergen-specific Th2 responses can be shifted to Th0/Th1 responses. Plasmacytoid dendritic cells (pDCs) produce large amounts of type I interferons (IFNs) after stimulation of Toll Like Receptor 9 (TLR9) and are likely to play an important role in the reorientation of these Th2 cells. The expression of CD32a on the cell surface of pDCs makes this cell type attractive for targeted delivery of antigen and TLR agonists to revert Th2 responses. Therefore we sought to determine the efficacy of targeted delivery of CpG-C ODN to CD32a on the ability of human and monkey pDCs to secrete inflammatory cytokines. Here we demonstrate that targeted delivery of 3'-biotinylated CpG-C to CD32a on pDC induced phenotypical maturation as determined by CD80, CD83 and CD86 expression. Furthermore, targeting both monkey and human pDCs strongly augmented the secretion of IFNalpha compared to the delivery of CpG-C in an untargeted fashion (p |
| Databáze: | OpenAIRE |
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