Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption
| Autor: | Mohamed Zidane, Monique Pommepuy, Pierre Pothier, Aoife M. Doyle, Evelyne Kohli, Fabienne Bon, Françoise S. Le Guyader, Dario DeMedici, Franco Maria Ruggeri, Alessandra Bertone, Silvia Crudeli, Francesco Maddalo, Marina Monini, Elisabetta Suffredini, Sylvain Parnaudeau, Anne Gallay |
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| Přispěvatelé: | Laboratoire de Microbiologie, IFREMER, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire Interactions Muqueuses Agents Transmissibles (LIMA), Université de Bourgogne (UB), Centro Nazionale per la Qualita` degli Alimenti e per i Rischi Alimentari, Azienda Unita` Sanitaria Locale, Azienda Unita` Sanitaria Locale, La Spezia, Dipartimento di Sanita` Alimentare e Animale, Istituto Superiore di Sanita`, Dipartimento di Sanita` Alimentare e Animale, Istituto Superiore di Sanita`, Rome, De´partement Maladies Infectieuses, Unite´ Infections Ente´riques, Alimentaires et Zoonoses (INVS), Institut de Veille Sanitaire (INVS), This study was partially supported by grants from the Italian Ministry of Health (Programma per la ricerca corrente anno 2000 [Metodi di prova per il controllo del rischio tossicologico e virologico nei prodotti ittici-IZSLER PRC 2000008]), the European Community, FBVE (QLK1-CT-1999-00594), EVENT (FP6-2002-SSP-1), and Virus Safe Seafood (QLK1-1999-00634)., Laboratoire de Microbiologie, IFREMER, Département Maladies Infectieuses, Nantes, Laboratoire Interactions Agents Pathogènes LIMA, Dijon, Centro Natzionale per Qualita degli Alimenti e per i Rischi Alimentari, Rome, Dipermento di Sanita Alimentare e Animale, Instituto Superiore di Sanita, Institut de Veille Sanitaire, Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Laboratoire Interactions Muqueuses Agents Transmissibles ( LIMA ), Université de Bourgogne ( UB ), De´partement Maladies Infectieuses, Unite´ Infections Ente´riques, Alimentaires et Zoonoses ( INVS ) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Oyster Epidemiology MESH : Aged MESH : Child Preschool medicine.disease_cause Disease Outbreaks Foodborne Diseases Feces MESH: Aged 80 and over MESH : Child [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Child 80 and over MESH : Female MESH: Animals MESH: Disease Outbreaks Child Aged 80 and over MESH: Aged 0303 health sciences MESH: Middle Aged biology Reverse Transcriptase Polymerase Chain Reaction MESH : Reverse Transcriptase Polymerase Chain Reaction food and beverages MESH: Feces MESH : Adult Middle Aged 3. Good health Gastroenteritis MESH : Gastroenteritis [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Child Preschool Gastroenteritis outbreak Female Microbiology (medical) Adult MESH: Norovirus Adolescent MESH : Male MESH: Ostreidae 03 medical and health sciences Contamination biology.animal Environmental health Virology MESH : Adolescent medicine Animals Humans MESH : Middle Aged MESH : Disease Outbreaks MESH : Foodborne Diseases MESH : Aged 80 and over Preschool MESH: Foodborne Diseases Shellfish 030304 developmental biology Aged MESH: Adolescent MESH : Ostreidae MESH: Humans 030306 microbiology MESH : Norovirus MESH : Humans Norovirus MESH: Child Preschool Outbreak MESH: Adult MESH : Feces biology.organism_classification Ostreidae MESH: Gastroenteritis MESH: Male Seafood MESH : Animals MESH: Female |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2006, 44 (11), pp.3878-82. ⟨10.1128/JCM.01327-06⟩ Journal of Clinical Microbiology, American Society for Microbiology, 2006, 44 (11), pp.3878-82. 〈10.1128/JCM.01327-06〉 Journal of Clinical Microbiology (0095-1137) (American Society for Microbiology.), 2006-11, Vol. 44, N. 11, P. 3878-3882 |
| ISSN: | 0095-1137 |
| Popis: | An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to rapidly connect the data and to fully interpret the results, since it was not obvious that one food could be the link because of the diversity of the several norovirus strains involved in the different cases. It was also demonstrated that heavy rain was responsible for the accidental contamination of seafood, leading to a concentration of up to hundreds of genomic copies per oyster as detected by real-time RT-PCR. |
| Databáze: | OpenAIRE |
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