Measuring the effects of postmortem time and age on mouse lens elasticity using atomic force microscopy
| Autor: | Marco Ruggeri, Wyndham More Batchelor, Sara Cabrera-Ghayouri, E. Arrieta-Quintero, Fabrice Manns, Bianca Maceo Heilman, Jean-Marie A. Parel, Mohammed I. Dibas, Noel M. Ziebarth |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Aging
Experimental control Lens Capsule Crystalline Mouse Lens Microscopy Atomic Force law.invention Mice Cellular and Molecular Neuroscience In vivo law Lens Crystalline medicine Animals Elasticity (economics) Chemistry Atomic force microscopy Presbyopia medicine.disease Elasticity Sensory Systems Lens (optics) Ophthalmology Models Animal Female sense organs Biomedical engineering |
| Zdroj: | Experimental Eye Research. 212:108768 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2021.108768 |
| Popis: | The mouse lens is frequently used both in vivo and ex vivo in ophthalmic research to model conditions affecting the human lens, such as presbyopia. The mouse lens has a delicate structure which is prone to damage and biomechanical changes both before and after extraction from the whole globe. When not properly controlled for, these changes can confound the biomechanical analysis of mouse lenses. In this study, atomic force microscopy microindentation was used to assess changes in the Young's Modulus of Elasticity of the mouse lens as a function of mouse age and postmortem time. Old mouse lenses measured immediately postmortem were significantly stiffer than young mouse lenses (p = 0.028). However, after 18 h of incubation, there was no measurable difference in lens stiffness between old and young mouse lenses (p = 0.997). This demonstrates the need for careful experimental control in experiments using the mouse lens, especially regarding postmortem time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect