Direct Molecular Detection and Genotyping of Borrelia burgdorferi Sensu Lato in Cerebrospinal Fluid of Children with Lyme Neuroborreliosis
| Autor: | Franziskus Johannes Bosse, Grete Vigemyr, Knut Øymar, Maryam Saeed, Ilka Huber, Sølvi Noraas, Ingvild S. Ask, Bjørn Barstad, Dag Tveitnes, Hanne Quarsten |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial Male 0301 basic medicine Microbiology (medical) Genotype 030106 microbiology Real-Time Polymerase Chain Reaction medicine.disease_cause Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Lyme disease Borrelia burgdorferi Group CSF pleocytosis 030225 pediatrics Borrelia parasitic diseases medicine Humans Lyme Neuroborreliosis Prospective Studies Borrelia burgdorferi Child Genotyping biology Norway business.industry Bacteriology bacterial infections and mycoses biology.organism_classification medicine.disease Antibodies Bacterial Molecular Diagnostic Techniques Child Preschool Immunology Female Borrelia garinii business Neuroborreliosis geographic locations |
| Zdroj: | Journal of Clinical Microbiology. 56 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The current diagnostic marker of Lyme neuroborreliosis (LNB), the Borrelia burgdorferi sensu lato antibody index (AI) in the cerebrospinal fluid (CSF), has insufficient sensitivity in the early phase of LNB. We aimed to elucidate the diagnostic value of PCR for B. burgdorferi sensu lato in CSF from children with symptoms suggestive of LNB and to explore B. burgdorferi sensu lato genotypes associated with LNB in children. Children were prospectively included in predefined groups with a high or low likelihood of LNB based on diagnostic guidelines (LNB symptoms, CSF pleocytosis, and B. burgdorferi sensu lato antibodies) or the detection of other causative agents. CSF samples were analyzed by two B. burgdorferi sensu lato -specific real-time PCR assays and, if B. burgdorferi sensu lato DNA was detected, were further analyzed by five singleplex real-time PCR assays for genotype determination. For children diagnosed as LNB patients (58 confirmed and 18 probable) ( n = 76) or non-LNB controls ( n = 28), the sensitivity and specificity of PCR for B. burgdorferi sensu lato in CSF were 46% and 100%, respectively. B. burgdorferi sensu lato DNA was detected in 26/58 (45%) children with AI-positive LNB and in 7/12 (58%) children with AI-negative LNB and symptoms of short duration. Among 36 children with detectable B. burgdorferi sensu lato DNA, genotyping indicated Borrelia garinii ( n = 27) and non- B. garinii ( n = 1) genotypes, while 8 samples remained untyped. Children with LNB caused by B. garinii did not have a distinct clinical picture. The rate of detection of B. burgdorferi sensu lato DNA in the CSF of children with LNB was higher than that reported previously. PCR for B. burgdorferi sensu lato could be a useful supplemental diagnostic tool in unconfirmed LNB cases with symptoms of short duration. B. garinii was the predominant genotype in children with LNB. |
| Databáze: | OpenAIRE |
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