A general method for the purification of restriction enzymes

Autor:	David J. Russel, Francisco Bolívar, Mary C. Betlach, Patricia J. Greene, Herbert W. Boyer, Robert C. Tait, Alejandra A. Covarrubias, Herbert L. Heyneker, Raymond L. Rodriguez, Keith Backman
Jazyk:	angličtina
Rok vydání:	1978
Předmět:	chemistry.chemical_classification General method Bacteria DNA Restriction Enzymes Biology Molecular cloning Chromatography Ion Exchange chemistry.chemical_compound Restriction enzyme Enzyme chemistry Biochemistry Genetics Chromatography Gel Physical mapping Restriction digest DNA
Popis:	An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9faf6865ade50822188596632da5f4e5 https://europepmc.org/articles/PMC342170/ Zobrazit plný text záznamu