Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?
| Autor: | Bing Yang, Aaron Singer, Elizabeth Kong, Tyler S. Williams, Mira V. Han, Martin R. Schiller, Ronald Benjamin, Ji Eun Bae, Sara G. Trimidal |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Computer science
Computational biology Genome General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Genome editing Transcription Activator-Like Effector Nucleases CRISPR Humans DNA Breaks Double-Stranded Indel 030304 developmental biology Gene Editing 0303 health sciences Transcription activator-like effector nuclease Cas9 fungi food and beverages DNA Zinc finger nuclease Zinc Finger Nucleases Meganuclease CRISPR-Cas Systems 030217 neurology & neurosurgery |
| Zdroj: | Bioessays |
| Popis: | Genome editing with engineered nucleases (GEENs) introduce site-specific DNA double-strand breaks (DSBs) and repairs DSBs via nonhomologous end-joining (NHEJ) pathways that eventually create indels (insertions/deletions) in a genome. Whether the features of indels resulting from gene editing could be customized is asked. A review of the literature reveals how gene editing technologies via NHEJ pathways impact gene editing. The survey consolidates a body of literature that suggests that the type (insertion, deletion, and complex) and the approximate length of indel edits can be somewhat customized with different GEENs and by manipulating the expression of key NHEJ genes. Structural data suggest that binding of GEENs to DNA may interfere with binding of key components of DNA repair complexes, favoring either classical- or alternative-NHEJ. The hypotheses have some limitations, but if validated, will enable scientists to better control indel makeup, holding promise for basic science and clinical applications of gene editing. Also see the video abstract here https://youtu.be/vTkJtUsLi3w. |
| Databáze: | OpenAIRE |
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