Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp
| Autor: | Harry Gruppen, Peter A. Wierenga, Anja Schwenzfeier |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Environmental Engineering
Immunoblotting Ion chromatography Color glycinin Centrifugation Bioengineering nitrogen Absorption Algae Chlorophyta expanded-bed adsorption Levensmiddelenchemie Protein purification chlorella Tetraselmis Solubility Waste Management and Disposal Plant Proteins VLAG marine microalgae Chromatography Food Chemistry biomass biology Renewable Energy Sustainability and the Environment Extraction (chemistry) General Medicine Hydrogen-Ion Concentration Chromatography Ion Exchange biology.organism_classification Chlorella hydrolysis Ionic strength cells chromatography Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet acid |
| Zdroj: | Bioresource Technology, 102(19), 9121-9127 Bioresource Technology 102 (2011) 19 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2011.07.046 |
| Popis: | Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64% (w/w) proteins and 24% (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100% solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range. |
| Databáze: | OpenAIRE |
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