End-to-End Platform for Human Pluripotent Stem Cell Manufacturing
| Autor: | Eytan Abraham, Inbar Friedrich Ben-Nun, Nicholas Uth, Puspa R. Pandey, Igor Ngabo, Yonatan Levinson, Haritha Vallabhaneni, Marites T. Woon, Amarel Tomney, Farjad Shafighi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type Computer science Induced Pluripotent Stem Cells Primary Cell Culture Catalysis Article Inorganic Chemistry 03 medical and health sciences microcarriers 0302 clinical medicine expansion End-to-end principle Cell harvest Bioreactor Humans Cellular Reprogramming Techniques Myocytes Cardiac human pluripotent stem cells Physical and Theoretical Chemistry Induced pluripotent stem cell Molecular Biology Spectroscopy Cells Cultured Cell Proliferation automation Automation Laboratory Organic Chemistry Microcarrier Monitoring system Cell Differentiation bioreactors General Medicine Expansion phase differentiation Computer Science Applications allogeneic cell therapy 030104 developmental biology Biochemical engineering 030217 neurology & neurosurgery kSep |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 1 |
| ISSN: | 1422-0067 |
| Popis: | Industrialization of stem-cell based therapies requires innovative solutions to close the gap between research and commercialization. Scalable cell production platforms are needed to reliably deliver the cell quantities needed during the various stages of development and commercial supply. Human pluripotent stem cells (hPSCs) are a key source material for generating therapeutic cell types. We have developed a closed, automated and scalable stirred tank bioreactor platform, capable of sustaining high fold expansion of hPSCs. Such a platform could facilitate the in-process monitoring and integration of online monitoring systems, leading to significantly reduced labor requirements and contamination risk. hPSCs are expanded in a controlled bioreactor using perfused xeno-free media. Cell harvest and concentration are performed in closed steps. The hPSCs can be cryopreserved to generate a bank of cells, or further processed as needed. Cryopreserved cells can be thawed into a two-dimensional (2D) tissue culture platform or a three-dimensional (3D) bioreactor to initiate a new expansion phase, or be differentiated to the clinically relevant cell type. The expanded hPSCs express hPSC-specific markers, have a normal karyotype and the ability to differentiate to the cells of the three germ layers. This end-to-end platform allows a large scale expansion of high quality hPSCs that can support the required cell demand for various clinical indications. |
| Databáze: | OpenAIRE |
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