Wolbachia in mosquitoes from the Central Valley of California, USA

Autor: Eunis Hernandez, Valeria Flores, Andrea L. Joyce, Jose Luis Ramirez, Ryan Torres
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
and promotion of well-being
Entomology
Mycology & Parasitology
Multilocus sequence typing (MLST)
California
Aedes aegypti
0302 clinical medicine
Aedes
RNA
Ribosomal
16S
Culex pipiens
2.2 Factors relating to the physical environment
Aetiology
Phylogeny
reproductive and urinary physiology
biology
Bacterial
Culiseta inornata
Bacterial Typing Techniques
Culex
Infectious Diseases
Medical Microbiology
Public Health and Health Services
Female
Wolbachia
Infection
Aedes melanimon
DNA
Bacterial
16S
030231 tropical medicine
Multilocus sequence typing
Zoology
Mosquito Vectors
Culex stigmatosoma
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Tropical Medicine
Anopheles
parasitic diseases
Animals
Supergroup
lcsh:RC109-216
16S rRNA
Ribosomal
3.2 Interventions to alter physical and biological environmental risks
Prevention
Research
fungi
Strain characterization
DNA
biochemical phenomena
metabolism
and nutrition
Prevention of disease and conditions
biology.organism_classification
16S ribosomal RNA
Vector control
Vector-Borne Diseases
Culicidae
030104 developmental biology
Parasitology
RNA
bacteria
Arthropod
Multilocus Sequence Typing
Zdroj: Parasites & Vectors, Vol 13, Iss 1, Pp 1-13 (2020)
Parasites & Vectors
Parasites & vectors, vol 13, iss 1
ISSN: 1756-3305
Popis: Background Wolbachia bacteria are widely distributed throughout terrestrial arthropod species. These bacteria can manipulate reproduction and influence the vector competence of their hosts. Recently, Wolbachia have been integrated into vector control programmes for mosquito management. A number of supergroups and strains exist for Wolbachia, and they have yet to be characterized for many mosquito species. In this study, we examined Wolbachia prevalence and their phylogenetic relationship to other Wolbachia, using mosquitoes collected in Merced County in the Central Valley of California. Methods Adult mosquitoes were collected from 85 sites in Merced County, California in 2017 and 2018. Traditional and quantitative PCR were used to investigate the presence or absence and the density of Wolbachia, using Wolbachia-specific 16S rRNA and Wolbachia-surface protein (wsp) genes. The supergroup of Wolbachia was determined, and Multilocus Sequence Typing (MLST) by sequencing five housekeeping genes (coxA, gatB, ftsZ, hcpA and fbpA) was also used to determine Wolbachia supergroup as well as strain. Results Over 7100 mosquitoes of 12 species were collected: Aedesmelanimon, Ae. nigromaculis, Ae. vexans, Ae. aegypti, Culex pipiens, Cx. stigmatosoma, Cx. tarsalis, Anopheles franciscanus, An. freeborni, An. punctipennis, Culiseta incidens and Cs. inornata. Eight showed evidence of Wolbachia. To our knowledge, this study is the first to report detection of Wolbachia in five of these species (Ae. melanimon, Cx. stigmatosoma, Cx. tarsalis, Cs. incidens and Cs. inornata). Culex pipiens and Cx. stigmatosoma had a high frequency and density of Wolbachia infection, which grouped into supergroup B; Cs. inornata clustered with supergroup A. MLST comparisons identified Cx. pipiens and Cx. stigmatosoma as wPip strain type 9 supergroup B. Six species had moderate to low (< 14%) frequencies of Wolbachia. Four species were negative, Ae. nigromaculis, An. franciscanus, An. freeborni and Ae. aegypti. Conclusions New records of Wolbachia detection were found in mosquitoes from Merced County, California. Culex stigmatosoma and Cs. inornata were new records for Wolbachia supergroup B and A, respectively. Other species with Wolbachia occurred with low frequency and low density. Detection of Wolbachia in mosquitoes can be used to inform potential vector control applications. Future study of Wolbachia within Cx. stigmatosoma and Cs. inornata in California and through the range of these species could further explore Wolbachia infection in these two species.
Databáze: OpenAIRE
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